食源蜡样芽孢杆菌16SrDNA分子鉴定及其抑菌活性的研究  

The Identification of 16S rDNA Molecules on Bacillus Cereus selected from Food Source and Preliminary Study on Antimicrobial Activity of It

作　　者：张美英[1] 何珊[1] 蔡琳[1] 石继飞[1] 

机构地区：[1]包头医学院医学技术学院,内蒙古包头014030

出　　处：《内蒙古师范大学学报（自然科学汉文版）》2015年第1期76-79,90,共5页Journal of Inner Mongolia Normal University(Natural Science Edition)

基　　金：内蒙古自然科学基金资助项目(2012MS1144)

摘　　要：对食源中筛选的具有抑菌活性、初步鉴定为蜡样芽孢杆菌的4株菌,进行16SrDNA分子鉴定,采用PCR扩增技术,建立4株菌的系统发育进化树,进行序列同源性分析,并通过琼脂打孔扩散法测定4株菌发酵液的抑菌谱.实验结果表明,4株菌16SrDNA基因序列基本一致,为同源蜡样芽孢杆菌,命名为BC2345.4株菌的发酵液具有较宽的抑菌谱,对金黄色葡萄球菌、铜绿假单胞菌和沙门氏菌等常见病原菌具有抑制作用.This study was on 16S rDNA molecular identification for the four bacteria strains screened out from the food source and the preliminary results of their antimicrobial spectrum in fermented liquid. After PCR amplification and sequencing analysis, the phylogenetic tree of the four bacteria strains was established. The results reveal that the 16S rDNA sequences of the four bacteria strains are basically consistent,which are the homologous of bacillus cereus named as BC2345. In addition, the antimicrobial spectrum of fermented liquid of the four bacteria strains was measured through the agar diffusion method and showed wider inhibitory effect on the common pathogenic bacteria such as staphylococcus aureus, pseudomonas aeruginosa,salmonella and so on.

关 键 词：食源 蜡样芽孢杆菌 16S rDNA分子鉴定 抑菌活性 

分 类 号：Q939.124[生物学—微生物学]