青海湖裸鲤体表白点病病原菌的分离与药敏试验  

Identification and Drug Sensitivity Test of Pathogenic Bacteria of White-spot Disease From Skin Surface of Gymnocypris przewaiskii

作　　者：李增魁[1] 李长忠[2] 康明[1] 圈华[1] 张勤文[1] 刘道鑫 马东红[1] 夏若措毛 李英[1] 

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大学生态环境工程学院,青海西宁810016

出　　处：《动物医学进展》2015年第4期32-36,共5页Progress In Veterinary Medicine

基　　金：青海省科技厅项目(2012-H-814 2;2011-N-517)

摘　　要：为确定养殖青海湖裸鲤(Gymnocypris przewaiskii)体表白点病的病原,分别对患病鱼白色点状囊泡样物进行了细菌分离、生理生化特性鉴定、16SrRNA基因分析和药敏感试验,分离到了以弗氏柠檬酸杆菌(Citrobacter freundii)、维氏气单胞菌(Aeromonas veronii,AV)和摩氏摩根菌(Morganella fulton)为代表的菌落形态一致、革兰阴性细菌;且分离到的3种菌都对头孢吡肟、丁胺卡那霉素、庆大霉素等药物敏感。To assure the pathogenic bacteria of white-spot disease from skin surface in Gymnocypris przewaiskii and treat the disease,three bacterial strains（Citrobacter freundii,Aeromonas veronii,Morganella fulton）were isolated from naturally infected Gymnocypris przewaiskii and identified by morphology,cultural characteristics,physical and chemical characters,and 16 SrRNA sequence analysis.The three strains were Gram negative.Colony morphologies were consistent.Drug sensitivity test revealed that the three strains were highly sensitive to cefepime,amikacin and gentamicin,which will benefit the treatment of disease in Gymnocypris przewaiskii.

关 键 词：青海湖裸鲤 弗氏柠檬酸杆菌 维氏气单胞菌 摩氏摩根菌 16SRRNA基因 药敏试验 

分 类 号：S859.7[农业科学—临床兽医学] S941.425[农业科学—兽医学]