牛磺酸对脂多糖诱导的小鼠肝脏损伤的缓解作用  

Mitigative Effect of Taurine on Lipopolysaccharides-Induced Liver Injury in Mice

作　　者：付凌[1] 宋剑波[1] 胡春燕[1] 曾黎明[1] 

机构地区：[1]江西农业大学,南昌330045

出　　处：《动物营养学报》2015年第3期989-996,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：江西农业大学博士科研启动基金(209004532)

摘　　要：本试验旨在研究牛磺酸对脂多糖(LPS)诱导的小鼠肝脏损伤的缓解作用。选用30只体重(22±3)g的ICR雄性小鼠,随机分为3组(每组10只小鼠,n=10):对照组和LPS组饲喂基础饲粮,牛磺酸组在基础饲粮中添加2.5%的牛磺酸。饲喂1周后LPS组和牛磺酸组小鼠腹腔注射10 mg/kg的LPS(LPS溶解于生理盐水,注射剂量为0.2 mL/只),对照组小鼠腹腔注射等体积的生理盐水。LPS注射24 h后,各组小鼠眼眶采血并处死,采集肝脏。血液用于血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性的测定,肝脏称重后检测氧化应激参数,抗氧化基因及转录因子E2相关因子2(Nrf2)、Kelch样ECH相关蛋白(Keap1)的相对表达量。结果表明:1)LPS处理显著升高了小鼠的肝脏指数(P<0.05),而添加牛磺酸显著抑制了由LPS引起的肝脏指数升高(P<0.05)。2)LPS处理显著增加了血清ALT和AST活性(P<0.05),添加牛磺酸后抑制了血清ALT和AST活性的增加,且其AST活性达到对照组水平(P>0.05)。3)LPS组小鼠肝脏出现明显的损伤,而牛磺酸组小鼠肝脏组织损伤较轻。4)与对照组相比,LPS组小鼠肝脏丙二醛(MDA)含量显著上升(P<0.05),谷胱甘肽过氧化物酶(GPx)活性显著降低(P<0.05),而牛磺酸组上述指标未发生显著变化(P>0.05)。5)牛磺酸显著缓解了LPS对肝脏GPx1和Nrf2表达的抑制作用(P<0.05)。综上所述,饲粮中添加2.5%的牛磺酸对LPS诱导的小鼠肝脏损伤起到了一定的缓解作用。This study aimed to investigate the mitigative effect of taurine on lipopolysaccharides （ LPS ）-in-duced liver injury in mice. Thirty ICR male mice with the similar body weight of （22＆#177;3） g were randomly di-vided into three groups （ ten mice in each group, n=10）： a control group and a LPS group in which mice re-ceived a basal diet, and a taurine group in which mice received the basal diet＋2.5% taurine. After fed 1 week, mice in the LPS group and taurine group were intraperitoneally injected 10 mg/kg LPS （ LPS was dissolved in physiological saline, and injection dose was 0.2 mL per mouse） and mice in the control group were intraperito-neally injected the same dose of physiological saline. Blood samples were harvested from eyes and all mice were killed at 24 h after injection to collect liver. Serum alanine aminotransferase （ ALT） and aspartate amin-otransferase （ AST） activities were measured and liver weight, oxidative stress parameters, and the relative ex-pression of antioxidant genes, nuclear factor E2-related factor 2 （ Nrf2） and Kelch like ECH associated protein 1 （ Keap1） were determined. The results showed as follows：1） LPS treatment significantly increased liver in-dex, while taurine supplementation significantly reduced the liver index to normal level （P〈0.05）. 2） LPS treatment significantly enhanced serum ALT and AST activities （ P〈0.05） , and taurine supplementation exhib-ited some beneficial effects on serum ALT and AST activities, and no significant difference in serum AST ac-tivity between taurine group and control group （P〉0.05）. 3） LPS caused marked liver injury in mice, and mice in taurine group suffered little liver injury. 4 ） Compared with control group, liver malondialdehyde （ MDA） content was significantly increased and live glutathione peroxidase （ GPx） activity was significantly decreased in LPS group （P〈0.05）, while those parameters in taurine group had no significant changes （P〉0.05） . 5） Taurine

关 键 词：牛磺酸 肝脏损伤 氧化应激 脂多糖 小鼠 

分 类 号：S816[农业科学—饲料科学] S852.4[农业科学—畜牧学]