骨形态发生蛋白2聚乳酸缓释微球对兔骨髓间充质干细胞相容性研究  

Bone morphogenetic protein 2 poly lactic acid sustained release microspheres for compatibility between rabbit bone marrow mesenchymal stem cell research

作　　者：马立坤[1] 邓江[1] 黄文良[1] 叶鹏[1] 田仁元[1] 吕雪峰[1] 

机构地区：[1]遵义医学院第三附属医院骨一科,贵州遵义563000

出　　处：《重庆医学》2015年第8期1022-1024,1028,共4页Chongqing medicine

基　　金：贵州省省长基金资助项目[2011(25)];贵州省科学技术基金资助项目(黔科合SY[2010]3101);贵州省社会公关基金资助项目(遵义市科合社字[2010]015号)

摘　　要：目的观察重组人骨形态发生蛋白2(rhBMP-2)聚乳酸(PLA)缓释微球对兔骨髓间充质干细胞(BMSCs)相容性。方法通过复乳干燥法制备rhBMP-2-PLA缓释微球,并与兔BMSCs共培养,采用MTT法检测rhBMP-2-PLA缓释微球对细胞的毒性及增殖,并通过倒置显微镜及扫描电镜等评价材料的细胞相容性。结果 rhBMP-2-PLA缓释微球各浓度浸提液与BMSCs培养得出对细胞无毒性。实验组与对照组4个不同时间细胞增殖光密度(OD)值经重复测量方差分析得出时间之间OD值比较,差异有统计学意义(P=0.000);实验组与对照组OD值比较差异有统计学意义(P=0.025),实验组OD值高于对照组,时间与组数有显著交互效应,二者变化趋势明显不同(P=0.006)。倒置显微镜观察材料组细胞增殖正常,扫描电镜发现接种7d后细胞周边有rhBMP-2-PLA缓释微球,细胞生长良好,增殖分裂活跃。结论 rhBMP-2-PLA缓释微球对BMSCs无毒性,具有良好的细胞相容性。Objective To investigate the influence of recombinant human bone morphogenetic protein‐2（rhBMP‐2）of poly lactic acid（PLA） release microspheres for compatibility of rabbit bone marrow mesenchymal stem cells（BMSCs） .Methods The rh‐BMP‐2‐PLA release microspheres were prepared by w/o/w multiple emulsion volatilizing method and then cocultured BMSCs .The effects of rhBM P‐2‐PLA release microspheres on the cytotoxicity and relative proliferation rate by MTTassay .Evaluation of mate‐rials biocompatibility by inverted microscope and scanning electron microscope（SEM） .Results The rhBMP‐2‐PLA release micro‐spheres in various concentration of leaching solution and BMSCs training of uninfected cells .Experimental group and control group in 4 different time cell proliferation OD values by analysis of repeated measurement variance between time OD values were statisti‐cally significant（P=0 .000） ,the experimental group and control group OD values are statistically significant（P=0 .025） ,the exper‐imental group higher than the control group ,experimental group OD value time there was a significant interaction effect and the group number ,the change trend are obviously different（P=0 .006） .Inverted microscope to observe materials normal cell prolifera‐tion ,SEM found that vaccination cells surrounding rhBMP‐2‐PLA release microspheres of 7 days later ,the cells grew well and split proliferation activity .Conclusion rhBMP‐2‐PLA release microspheres of BMSCs has non‐toxic and has compatibility .

关 键 词：RHBMP-2 PLA 缓释微球 骨髓间充质干细胞 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程]