RFA联合TACE和HFH对兔VX2肝移植瘤细胞凋亡的影响  

Effect of radiofrequency ablation combined with trans arterial chemoembolization and high-frequency hyperthermia on apoptosis in rabbit liver VX2 carcinoma

作　　者：乔正荣[1] 付志豪[1] 冯虎翼[1] 李元君[1] 龚建平[1] 李生伟[1] 

机构地区：[1]重庆市第五人民医院普外科,400062

出　　处：《重庆医学》2015年第8期1025-1028,共4页Chongqing medicine

基　　金：重庆市卫生局重点基金资助项目(渝卫2010-1-68);重庆市南岸区科技攻关基金资助项目(南科发2009-12)

摘　　要：目的探讨射频消融(RFA)联合经动脉化疗栓塞(TACE)和高频热疗(HFH)治疗对兔VX2肝移植瘤细胞凋亡的影响。方法建立兔VX2肝移植瘤模型,随机分为RFA+TACE治疗组(A组),RFA+TACE+HFH治疗组(B组),RFA+HFH治疗组(C组)和TACE+HFH治疗组(D组)。检测各组治疗后的血清谷丙酸转氨酶(ALT),了解治疗的安全性。TUNEL法检测癌组织凋亡及计算凋亡指数;免疫组织化学、Western blot和实时荧光定量PCR技术检测Caspase-3蛋白和mRNA水平。结果血清ALT在B组升高最明显。与其他组比较,凋亡指数在B组显著升高(P<0.05)。免疫组织化学法表明Caspase-3蛋白在各组中出现强弱不同的表达,在B组表达明显增强。Western blot和实时荧光定量PCR显示Caspase-3蛋白和mRNA在B组表达最高,与其他组比较差异有统计学意义(P<0.05)。结论 RFA+TACE+HFH治疗能有效杀伤肿瘤细胞,促进肿瘤细胞的凋亡,但对于肝功能的损害也较为严重。Objective To study the effects of apoptosis of the tumor cells in rabbit liver VX2 carcinoma after treatment by radiofrequency ablation（RFA） combined with trans arterial chemoembolization（TACE） and high‐frequency hyperthermia（HFH） . Methods Rabbit liver VX2 carcinoma model was established .Rabbit liver VX2 tumor models were divided into the following group：group A ,RFA＋ TACE;group B ,RFA ＋ TACE ＋ HFH ;group C ,RFA ＋ HFH ;group D ,TACE＋ HFH .The changes of serum ALT was detected to realize the safety of the treatment .Cell apoptosis were detected by DNA agarose gel electrophoresis and terminal deoxynucleotidyltransferase‐mediated Dutp nick end‐labeling（TUNEL） assay ;SP immunohistochemistry ,Western blot and Real‐time quantitative PCR（RT‐PCR） were used to detect Caspase‐3 protein and mRNA expression levels .Results The changes of serum ALT in group B was significantly higher .Compared with other groups ,the apoptosis index in group B was increased marked‐ly（P〈0 .05） .Western blot and RT‐PCR Caspase‐3 protein and mRNA levels in group B were higher than the other groups（P〈0 .05） .Conclusion RFA＋ TACE＋ HFH can effectively kill tumor cells and promote apoptosis of tumor cells ,but ,at the same time ,can damage liver function .

关 键 词：肝癌 CASPASE-3 凋亡 射频消融 

分 类 号：R735.7[医药卫生—肿瘤]