肝硬化大鼠不同脏器NO表达差异及其机制  

Expression Differences of NO in Different Organs of Cirrhotic Rats and its Mechanism

作　　者：王黎敏[1] 张丽丽[2] 李旭炯[3] 陈云霞[4] 田小霞[2] 范毅敏[1] 张慧英[2] 

机构地区：[1]长治医学院机能综合实验室,046000 [2]长治医学院病理生理学教研室 [3]长治医学院生理学教研室 [4]长治医学院微生物学教研室

出　　处：《长治医学院学报》2015年第1期1-4,共4页Journal of Changzhi Medical College

基　　金：国家自然科学基金资助项目(81070339);山西省国际科技合作计划资助项目(2010081068);山西医科大学细胞生理学省部共建教育部重点实验室主任基金资助项目(2010-09);山西省回国留学人员科研基金资助项目(211-091)

摘　　要：目的:观察肝硬化大鼠发病过程中不同脏器NO表达的差异并探讨其可能原因。方法:采用复合致病因素法建立肝硬化大鼠模型。8周末测定大鼠血浆内毒素(ET)、肿瘤坏死因子-α(TNF-α)、丙氨酸氨基转移酶(ALT)水平;检测各组织匀浆中丙二醛(MDA)、一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)含量;组织学切片HE染色进行巨噬细胞计数。结果:与正常对照组相比,肝硬化大鼠血浆ET、TNF-α、ALT水平明显升高,肝、肺、心、脑、肾组织中巨噬细胞计数及匀浆中MDA、NO、iNOS含量升高,除脑组织巨噬细胞计数以外,其它指标升高均具有统计学意义。结论:肝硬化发病过程中,肠源性内毒素及其诱导产生的TNF-α,是不同脏器NO表达差异的重要原因;而巨噬细胞在各个脏器分布的特点,是导致NO表达差异的基本机制。Objective：To observe the expression of NO,and to explore their possible mechanism of expression difference in main organs of cirrhotic rats.Methods：The Cirrhotic rats model was established with multiple pathogenic factors.At the8 th weekend,endotoxin（ET）,tumor necrosis factor-α（TNF-α）,alanine aminotransferase（ALT）in plasma and malondialdehyde（MDA）,nitric oxide（NO）and inducible nitric oxide synthase（iNOS）in different tissues were detected.Macrophages were numbered in main organs with HE staining.Results：The cirrhotic rats models were successfully established.Compared with normal control group,the levels of ET,TNF-αand ALT in plasma were significantly higher,and macrophage numbers and the contents of MDA,NO and iNOS in tissues of liver,lung,heart and kidney were significantly increased.Conclusion：Intestinal endotoxemia and resultant TNF-αis basic cause of differences in the expression of NO,and the different distribution of macrophage in different organs is fundamental mechanism during the development of liver cirrhosis in rats.

关 键 词：肝硬化 内毒素 巨噬细胞 一氧化氮 氧化应激 

分 类 号：R363[医药卫生—病理学]