CADM1在大肠癌细胞系中的表达及与启动子甲基化的关系  

The correlation between CADM1 expression and promoter methylation in the colorectal cancer cell lines

作　　者：钟亮玉[1] 蒋永洪 陈科全[1] 

机构地区：[1]广州医科大学附属第一医院消化内科,广州510120

出　　处：《广东医学》2015年第3期336-338,共3页Guangdong Medical Journal

基　　金：广东高校优秀青年创新人才培养计划项目(编号:LYM11103)

摘　　要：目的探讨CADM1在大肠癌细胞系中的表达及与启动子甲基化的关系。方法用RT-PCR法检测大肠癌细胞系中CADM1基因m RNA表达水平,Western blot法检测细胞系CADM1蛋白表达情况,BSP法和MSP法检测CADM1基因启动子甲基化状态。结果 CADM1 m RNA在24%大肠癌细胞系表达缺失,在50%细胞系中CADM1 m RNA表达减少。75%大肠癌细胞系检测到CADM1基因启动子甲基化阳性,且表达缺失。结论 CADM1基因启动子甲基化与大肠癌的发生发展有密切关系,该基因的甲基化检测可作为癌症早期筛选、监测预后的肿瘤标志物。Objective To detect the promoter methylation and expression of CADM 1 gene in colon cancer cells . Methods RT -PCR was used to supervise the CADM1 gene expression level of mRNA .Western blotting was applied to validate the expression of CADM1 protein.BSP and MSP were employed to detect the methylation status of CADM1 gene promoter.Results CADM1 mRNA negative was detection in 24% of colon cancer cell lines , while down -regulated CADM1 mRNA expression was observed in 50% of cell lines.Methylation of CADM1 promoter and CADM1 expression loss were revealed in 75% of colorectal cancer cell lines .Conclusion The methylation of CADM1 promoter is closely related to pathogenesis of colon cancer , providing a probable tumor mark for early screening and prognosis prediction .

关 键 词：CADM1 启动子甲基化 大肠癌 

分 类 号：R737.14[医药卫生—肿瘤]