乳腺浸润性导管癌中SATB1和BRMS1的表达及与淋巴转移的相关性  

The expression of SATB1 and BRMS1 in breast invasive ductal carcinoma and their correlations with lymphatic metastasis

作　　者：张英[1] 陈丽娟[2] 刘永源[3] 

机构地区：[1]广东医学院附属医院肿瘤中心,广东湛江524001 [2]广东省东莞市人民医院乳腺科,523000 [3]广东医学院附属医院普外科,广东湛江524001

出　　处：《广东医学》2015年第4期532-537,共6页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2010B031600284)

摘　　要：目的探讨核基质结合区结合蛋白-1(SATB1)及乳腺癌转移抑制基因1(BRMS1)在乳腺浸润性导管癌(IDC)原发灶、腋窝淋巴结转移灶中的表达,分析SATB1与临床病理参数的关系及两者表达的相关性。方法采用免疫组化SP法及qRT-PCR检测51例乳腺IDC组织、17例对应腋窝淋巴结转移灶组织、32例配对癌旁乳腺组织中SATB1、BRMS1蛋白及mRNA的表达情况。结果在原发灶组织、腋窝淋巴结转移灶组织SATB1阳性表达率分别是76.47%、82.35%,均高于癌旁组织的28.12%(P<0.05);BRMS1蛋白的表达率分别是25.49%、23.53%,均低于于癌旁组织的87.50%(P<0.05);在原发灶组织、腋窝淋巴结转移灶组织SATB1 mRNA表达量分别是癌旁组织的3.88、6.06倍;BRMS1 mRNA表达量分别是癌旁组织的0.36、0.35倍,差异均有统计学意义(P<0.05);SATB1蛋白及mRNA、BRMS1 mRNA在腋窝淋巴结有转移的乳癌原发灶中的表达与腋窝淋巴结无转移乳癌原发灶间存在明显差异(P<0.05);在原发灶和淋巴结转移灶中的表达差异无统计学意义(P>0.05)。SATB1蛋白和mRNA表达与腋窝淋巴结转移数目、临床分期有关(P<0.05),与患者的年龄、肿瘤大小、肿瘤病理学分级、雌激素受体、孕激素受体、人表皮生长因子受体2、增殖细胞核抗原(Ki-67)及绝经状态无关(P>0.05)。相关分析结果显示在原发灶组织、腋窝淋巴结转移组织中SATB1、BRMS1蛋白及mRNA表达均无相关性(P>0.05)。结论 SATB1、BRMS1异常表达与IDC发生、发展、淋巴转移有密切关系,SATB1有望成为评估乳腺IDC淋巴结转移潜能的分子标记物。Objective To investigate the expression of SATB 1 and BRMS1 in the tissue of the primary breast in-vasive ductal carcinoma （ IDC） , axillary nodes metastases and the normal breast tissues adjacent to carcinoma ;thus to an-alyze the correlation between SATB1 and clinicopathologic parameters , and the correlation between SATB1 and BRMS1. Methods The mRNA and proteins levees of SATB1 and BRMS1 in 51 samples of IDC tissues,17 samples of axillary nodes metastases tissues and 32 samples of the normal breast tissues adjacent to carcinoma tissues were detected by quanti -tative reverse transcription-polymerase chain reaction （ qRT-PCR） and immunohistochemical SP .Results The positive rates of SATB1 in IDC tissues and axillary nodes metastases tissues were 76.47%and 82.35%, respectively;significantly higher than that （28.12%） in the normal breast tissues adjacent to carcinoma （P〈0.05）.The positive rates of BRMS1 protein in IDC tissues and axillary nodes metastases tissues were 25.49%and 23.53%, respectively; significantly lower than that （87.5%） in the normal breast tissues adjacent to carcinoma （P〈0.05）.The levels of SATB1 mRNA were sig-nificantly 3.88-fold and 6.06-fold higher in IDC tissues and axillary nodes metastases tissues , respectively , than that in the normal breast tissues adjacent to carcinoma （P〈0.05）.The levels of BRMS1 mRNA were significantly 0.36-fold and 0.35-fold lower in IDC tissues and axillary nodes metastases tissues , respectively , than that in the normal breast tis-sues adjacent to carcinoma （ P〈0.05 ） .There was no significant difference found between the IDC tissues and axillary nodes metastases tissues （ P〉0.05） .There were significant differences in SATB 1 protein and mRNA and BRMS1 mRNA between the group of IDC with lymph node metastasis and the group of IDC without lymph node metastasis （ P〈0.05 ） . The expression of SATB1 were significantly correlated with axillary nodes metastases and clinical stage , but not with age , tumor size, path

关 键 词：SATB1 BRMS1 乳腺浸润性导管癌 淋巴转移 

分 类 号：R737.9[医药卫生—肿瘤]