整合素靶向性超顺磁性纳米分子探针体外显像人肝癌细胞的实验研究  

Integrin receptor targeted cRGD-conjugated magnetic nanoparticles for vitro imaging experimental hepatic cancer cells

作　　者：魏新华[1] 陈婧[1] 杨蕊梦[1] 张桂香[1] 肖云华[2] 任继亮[1] 徐向东[1] 江新青[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院放射科,广州510180 [2]广州医科大学附属广州市第一人民医院药剂科,广州510180

出　　处：《中华临床医师杂志（电子版）》2015年第5期68-71,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：广东省科技计划项目(粤科规划字[2011]97-序号1);广州市科技计划项目(2014J4100071)

摘　　要：目的构建环状精氨酸-甘氨酸-天冬氨酸多肽(c RGDf K)超顺磁性氧化铁纳米分子探针,并检测其对高表达αVβ3整合素受体的人肝癌Hep G2细胞的显像能力。方法 SPIO氨基化后与c RGDf K偶联生成c RGDf K-SPIO,在10μg/ml、20μg/ml、30μg/ml、40μg/ml、50μg/ml五种不同含铁浓度下对肝癌Hep G2细胞进行标记后行MR成像,观察在T2 mapping上T2弛豫时间的变化,找出最佳成像浓度。结果人肝癌Hep G2细胞在5种不同浓度c RGDf K-SPIO对比剂中培养24h后比较,各组之间的T2弛豫时间数值差异有统计学意义(F=336.389,P<0.01),各实验组与空白对照组两两比较,弛豫时间数值差异有统计学意义(P<0.01);T2值下降最大的为20μg/ml组(P<0.01)。结论 c RGDf K-SPIO可通过MRI靶向显示高表达整合素αVβ3受体的肝癌Hep G2细胞。本研究所制备的c RGDf K-SPIO分子探针在肝癌Hep G2细胞的体外最佳成像浓度是20μg/ml。ObjectiveTo construct a magnetic molecular probe named cRGDfK-SPIO for imaging experimental liver carcinoma cells specifically.MethodsThe cRGDfK-SPIOs were fabricated with cRGDfK peptide and SPIO through chemical method. The HepG2 cells were cultivated with cRGDfK-SPIO in different concentrations of Fe （10μg/ml, 20μg/ml, 30μg/ml, 40μg/ml, 50μg/ml） and imaged in MR. The HepG2 cells cultivated with cRGDfK were set as control group. The differences of T2 relaxation time between different concentrations in two groups were compared.ResultsThe HepG2 cells incubated with cRGDfK-SPIO showed different relaxation time in T2mapping imaging at different time points in experiment group （F=336.389,P〈0.01）, and the T2 relaxation time decreased significantly in 20μg/ml in vitro. The difference T2 relaxation time between two groups was statistical significant （P〈0.01）.ConclusionThe cRGDfK-SPIO could specifically image HepG2 cells with αVβ3 integrin receptorin vitro. And the best imaging concentrate of Fe in cRGDfK-SPIO is 20μg/ml.

关 键 词：分子探针 整合素ΑVΒ3 磁共振成像: 癌 肝细胞 

分 类 号：R735.7[医药卫生—肿瘤]