GrB/Bid蛋白在胃癌中表达及临床意义的初步研究  

The preliminary study of expression and clinical significance of GrB/Bid in gastric carcinoma

作　　者：任磊[1] 刘春凤[2] 周业江[1] 

机构地区：[1]泸州医学院附属医院胃肠外科,泸州646000 [2]泸州医学院附属医院呼吸内科,泸州646000

出　　处：《国际外科学杂志》2015年第1期23-26,73,共5页International Journal of Surgery

基　　金：国家自然基金资助项目（No.30672058）

摘　　要：目的 探讨胃癌组织中颗粒酶B（GrB）和Bid蛋白的表达情况及与胃癌演进的关系,了解二者在肿瘤免疫耐受机制中的作用.方法 采用免疫组织化学S-P法检测GrB和Bid在胃癌和切缘组织中的表达状况.结果 GrB在胃癌组织中阳性表达率为47.62％,切缘组织中的阳性率为40.48％,二者相比差异无统计学意义（P ＞0.05）;Bid在胃癌组织中的阳性表达率（26.19％）明显低于胃切缘上皮组织（76.19％）,二者相比有统计学意义（P ＜0.05）;GrB的表达水平与胃癌临床分期、淋巴转移有关（P＜0.05）,Bid的表达水平与胃癌分化程度、浸润深度、临床分期及淋巴结转移有关（P ＜0.05）;GrB和Bid在42例胃癌中表达呈负相关（rs=-0.311,P＜0.05）.结论 胃癌组织中GrB和Bid表达可能共同参与胃癌演进,胃癌组织中Bid的表达下调或缺失可能引起胃癌细胞逃逸内源或外源性GrB介导的细胞凋亡,获得免疫耐受潜能,逃逸机体免疫应答.Objective To investigate the expression of GrB and Bid gene in gastric carcinoma and development of gastric carcinoma,exploring their effect on the mechanism of immune tolerance of tumor.Methods S-P method was used to detect the expression status of GrB and Bid gene in gastric carcinoma and margin tissue.Results The positive expression rate of GrB of gastric carcinoma tissues was 47.62%,the positive rate in the margin tissue was 40.48%,there was no significant difference（P 〉 0.05） ; The positive expression rate of Bid of gastric carcinoma tissues （26.19%） was significantly lower than that of margin tissue（76.19%） （P 〈 0.05） ; The expression of GrB had significantly correlation with TNM stage and lymph node metastasis of the tumor （P 〈 0.05）,the expression of Bid in gastric cancer had significantly correlation with differentiation,invasive depth,TNM stage and lymph node metastasis of the tumor （P 〈 0.05） ; the expression of GrB was negatively correlated with that of Bid in gastric cancaer（rs =-0.311,P 〈 0.05）.Conclusion The expression of GrB and Bid may be involved in development of gastric carcinoma,down-regulated or scarce expression of Bid may be cause cancer cells escaping from endogenous or exogenous GrB-mediated cell apoptosis,and acquire immune tolerance,potential,to escape the immune response.

关 键 词：颗粒酶类 BID 胃肿瘤 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]