乳酸片球菌素Ped-A基因表达载体的构建  

Construction of Expression Vectors for Lactic Acid Pediocin Ped-A

作　　者：穆熙军[1] 刘秀侠[1] 许燕侠[1] 孙学森[1] 谷巍[1] 

机构地区：[1]山东宝来利来生物工程股份有限公司,山东泰安271000

出　　处：《饲料博览》2015年第2期1-5,共5页Feed Review

摘　　要：乳酸片球菌素可以作为防腐剂延长食品的保鲜期,为研究和开发可食用级别的防腐剂,将乳酸片球菌素结构基因Ped-A全长和不含信号肽的序列克隆至乳酸菌表达载体p MG36e和p NZ8048,经酶切鉴定及序列分析,所转化的大肠杆菌DH5α和DHB4中含有插入Ped-A基因全长和不含信号肽的序列的重组质粒。结果表明,成功构建了乳酸片球菌素Ped-A基因的乳酸菌表达载体p MG36e/Ped-A(全长/片段)、p NZ8048/Ped-A(全长/片段)。Lactic acid pediocin can be used as a preservative to extend the shelf life of food. The full gene se- quence of Ped-A and without signal peptide were inserted into expression vectors pMG36e and pNZ8048. By using re- striction enzyme and sequence analysis, it can be proved that recombinant plasmids pMG36e/Ped-A （full length / frag- ment） and pNZ8048/Ped-A （full length / fragment） were transformed into E. coli DH5α and DHB4 successfully. The result confirmed that Ped-A gene expression vectors were constructed successfully.

关 键 词：乳酸菌表达载体Ped-A 构建 乳酸菌表达载体 构建 

分 类 号：TS201.3[轻工技术与工程—食品科学]