免疫炎症反应在颅内动脉瘤形成中的作用  

Role of immune inflammation in the formation of intracranial aneurysm

作　　者：何安邦[1] 周奋[1] 潘德岳[1] 云望[1] 乔卫东[1] 江振忠[1] 曾坚锋 

机构地区：[1]海南省农垦三亚医院神经外科,三亚572000

出　　处：《国际脑血管病杂志》2015年第2期107-110,共4页International Journal of Cerebrovascular Diseases

摘　　要：目的：探讨免疫炎症反应在颅内动脉瘤形成中的作用。方法收集40例开颅手术患者颅内动脉瘤（颅内动脉瘤组）和20例开颅手术颅脑外伤患者（对照组）血管标本。应用荧光定量聚合酶链反应检测动脉壁白细胞介素（interleukin, IL）-17受体表达水平，流式细胞仪检测外周血Th17细胞，酶联免疫吸附法测定外周血IL-17以及动脉壁IL-6和肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）水平。结果颅内动脉瘤组年龄[（62.6±8.7）岁对（61.4±7.9）岁；t=0.342，P=0.681]、男性（60.0％对65.0％；χ2＝0.246， P=0.434）以及高血压（12.5％对10.0％；χ2＝0.315， P=0.492）、糖尿病（7.5％对10.0％；χ2＝0.284， P=0.482）和吸烟（35.5％对30.0％；χ2＝0.224， P=0.413）患者构成比均与对照组差异无统计学意义。对照组动脉壁IL-17受体表达（0.106±0.032对0.264±0.071；t=5.115，P=0.001）以及外周血T h17细胞比例[（2.75±0.53）％对（7.18±1.54）％；t=8.436， P〈0.001]和IL-17水平[（7.32±1.82）μg/L对（22.64±4.51）μg/L；t=8.357，P〈0.001]均显著低于颅内动脉瘤组。此外，对照组动脉壁 IL-6[（1.15±0.24）μg/L对（19.64±4.16）μg/L；t=9.527，P〈0.001]和TNF-α[（1.43±0.31）μg/L对（26.17±4.32）μg/L；t=9.816，P〈0.001]水平均显著低于颅内动脉瘤组。结论颅内动脉瘤患者动脉壁IL-17受体表达以及外周血T h17细胞比例和IL-17水平增高。免疫炎症反应可能与颅内动脉瘤的形成有关。ObjectiveToinvestigatetheroleofimmuneinflammatoryreactionintheformationof intracranial aneurysm. Methods The intracranial aneurysms in 40 patients of craniotomy （ intracranial aneurysm group） and the vascular specimens in 20 craniotomy patients w ith traumatic brain injury （control group） w ere col ected. Fluorescence quantitative polymerase chain reaction w as used to detect the expression of interleukin （IL）-17 receptor in the arterial w al . Flow cytometry w as used to detect the Th-17 cel s in peripheral blood. Enzyme-linked immunosorbent assay w as used to measure the levels of IL-17, IL-6 in the arterial w al and tumor necrosis factor-α（ TNF-α） in peripheral blood. Results There w ere no significant differences in the age （62.6 ±8.7 years vs.61.4 ±7.9 years;t=0.342;P=0.681）, proportions of male （60.0%vs.65.0%; χ2 =0.246, P=0.434）, hypertension （ 12.5%vs.10.0%; χ2 =0.315, P=0.492）, diabetes （75.0%vs.10.0%; χ2 =0.284, P=0.482）, and smoking （35.5%vs.30.0%; χ2 =0.224, P=0.413） betw een the intracranial aneurysms group and the control group. The expression of IL -17 receptor in the arterial w al （0.106 ±0.032 vs.0.264 ±0.071; t=5.115, P=0.001） and the proportion of Th17 cels in peripheral blood （2.75%±0.53%vs.7.18%±1.54%; t=8.436, P〈0.001） and IL-17 level （ 7.32 ±1.82 μg/L vs.22.64 ±4.51 μg/L; t= 8.357, P〈 0.001 ） in the control group w ere significantly low er than those in the intracranial aneurysm group. The levels of IL-6 （1.15 ±0.24 μg/L vs. 19.64 ±4.16 μg/L; t=9.527, P〈0.001） and TNF-α（1.43 ±0.31 μg/L vs.26.17 ±4.32 μg/L; t=9.816, P〈0.001） in the arterial wal in the control group were significantly lower than those in the intracranial aneurysm group. Conclusions The expression of IL-17 receptor in the arterial w al , the proportion of the Th17 cels and IL-17 level in peripheral blood were increased in patients with intracranial aneurysms. Immune inflammation may be involved in the formation of intracranial aneurysm.

关 键 词：颅内动脉瘤 炎症 白细胞介素-17 受体 白细胞介素-17 TH17细胞 INTERLEUKIN-17 Receptors Interleukin-17 

分 类 号：R651.1[医药卫生—外科学]