MiR-10a在急性髓系白血病不同FAB亚型中的表达及与耐药关系的研究  

MicroRNA-10a Expression in FAB Different Subtype of Acute Myeloid Leukemia and Its Relationship with Drug Resistance

作　　者：支勇金 支枫[2] 王榕[2] 薛莲[2] 顾伟英[1] 王彪[1] 董伟民[1] 李海乾[1] 凌云[1] 邱国强[1] 王志林[1] 曹祥山[1] 刘琰[1] 

机构地区：[1]苏州大学附属第三医院血液科,江苏常州213003 [2]苏州大学附属第三医院现代医学研究中心,江苏常州213003

出　　处：《中国实验血液学杂志》2015年第1期29-33,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金(81300422)

摘　　要：目的:探讨miR-10a在FAB不同亚型急性髓系白血病(AML)中的表达及其与AML耐药性的关系。方法:应用实时定量聚合酶链反应(RT-PCR)方法检测miR-10a在急性髓系白血病细胞株HL-60、U937、HL-60/ADR(阿霉素耐药株)、40例初治AML患者及16例非恶性血液病患者骨髓细胞中的表达,并分析其与AML临床特征的相关关系。结果:miR-10a在HL-60细胞株中的表达高于U937细胞株中的表达(P<0.05),在初治AML患者中的表达高于非恶性血液病组中的表达(P<0.01),在AML M3型患者中的表达高于M1、M2、M4型中的表达(P<0.05),在HL-60/ADR耐药株中的表达明显高于敏感株HL-60中的表达(P<0.01);除M3型外,经一疗程标准方案诱导化疗后未完全缓解组的miR-10a表达高于完全缓解组中的表达(P<0.01)。结论:miR-10a的高表达可能与早幼粒细胞的过度增生有关,并且与非M3型的急性髓系白血病细胞的耐药密切相关。Objective： This study was to investigate the expression of miR-10a in the different FAB subtype of acute myeloid leukemia （AML） and its relationship with drug resistance. Methods： Forty de novo patients with AML, 16 patients with non-malignant hematologic disease and three AML cell lines HL-60, U937 and HL-60/ADR were enrolled in this study, the MiR-10a expression in bone marrow mononuclear cells of above-mentioned patients and 3 AML cell lines was detected by TaqMan RT-PCR. The correlation of rniR-10a with clinicopathological factors of AML patients was analyzed. Results：The miR-10a expression level in HL-60 cell line was higher than that in U937 cell line （P = 0.039）. And its expression level in de novo AML patients was higher than that in patients with non-malignant hematologic disease （P 〈0.01 ）. FAB-AML-M3 patients exhibited higher expression of miR-10a than that in M1, M2 and M4（P 〈0.05） ; HL-60/ADR cell line showed higher miR-10a expression than that in HL-60 cell line （ P 〈 0.01 ）. Except M3, the patients without CR（non-CR） after the first cycle of chemotherapy showed a higher level of miR-10a as compared with CR patients（ P 〈 0.01 ）. Conclusion：The high expression of miR-10a may be closely related to over- proliferation of promyelocyte and drug resistance of acute myeloid leukemia cells, except M3.

关 键 词：miRNA-10a 白血病 髓系 急性 耐药 

分 类 号：R733.71[医药卫生—肿瘤]