SCID小鼠-人白血病模型中关键因素的分析  

Analysis of the Key Factors in SCID Mouse-Human Leukemia Model

作　　者：付林林[1] 陈日玲[2] 赵桂娟[1] 田川[2] 

机构地区：[1]即墨市人民医院,山东青岛266200 [2]广东医学院附属医院,广东湛江524000

出　　处：《中国实验血液学杂志》2015年第1期55-60,共6页Journal of Experimental Hematology

基　　金：广东省科技计划项目(2011B031800211)

摘　　要：目的:通过对白血病建模过程中生命状态不好的小鼠进行分析,探讨白血病建模的关键因素,为以后提高建模成功率提供理论参考。方法:SCID小鼠接种白血病细胞后1周,观察生命活动状态、检测外周血象,并对造模过程中频死/死亡小鼠与正常对照组小鼠进行解剖,检测骨髓涂片、脾脏病理切片、脾脏指数。结果:实验组小鼠相对对照组生命活动状态差,其血涂片中有幼稚细胞,白细胞稍多、多形态不规则且部分破裂,淋巴细胞和杆状核细胞明显增多,中性粒细胞核左移;骨髓涂片较对照组细胞体积较大,核质比较小,细胞以及细胞核形态异常,细胞破裂严重;小鼠脾脏不同程度肿大充血、脾脏指数明显升高。实验组脾脏病理切片较对照组脾脏间质扩张,细胞排列紊乱,细胞形态不规则,但两组均未见白血病细胞浸润。结论:建模过程中小鼠的周龄、接种白血病细胞的途径、饲养过程中无菌的条件、以及如何避免建模过程中排斥反应与炎症反应的发生均是影响建模成功与否的关键因素。Objective：This study was to explore the key factors in leukemia model through the analysis of mouse with bad life state in the modeling process of leukemia so as to provide the theoretical reference for improving the success rate of modeling. Methods ： At 1 week after inoculation of leukemia cells into SCID mice, the life status and peripheral hemogram of SCID mice were tested, the bone marrow smears, splean biopsy and spleen index of mice were examined after dissecting mormal and agoned/died mice during modoling, and the examined results were compared. Results ：As compared with control mice, the life status of experimental mice was poor; the blood smear test showed juvenile cells, slightly more white blood cells with irregular shape and partial rupture, the lymphocytes and hand cells obviously increased, the neutrophile granulocytes showed nuclear left shift; the bone marrow smears showed larger cell volume, smaller mulcoplasm, abnormal morphology of cells and cell nuclei and serious cell rapture; the spleen examination showed that the spleen diplayed enlargement and hyperemia to varying degree, the spleen index obviously increase, the spleen interstitial expansion, cell disordered arragement and irregular cell shope were observed, however there was no infiltration of leukemia cells in control and experimental mice. Conclusion： The mouse age, pathway of inoculating the leukemia cells, sterile condition in breading and avoiding the rejection and inflammatory response in modeling process are the key foctors influencing the modeling success.

关 键 词：急性白血病模型 SCID小鼠 动物模型 

分 类 号：R733.71[医药卫生—肿瘤]