HIF-lα@Fe304纳米颗粒标记对胰腺癌干细胞增殖及凋亡的影响  

Effect of HIF-lα @ Fe304 nanoparticles tag on proliferation and apoptosis of pancreatic cancer stem cells

作　　者：谢晓东[1] 朱海涛[1] 吴荧荧 黄文斯 王颖[1] 吴恺迪 张礼荣[1] 王冬青[1] 

机构地区：[1]江苏大学附属医院影像科,镇江212001

出　　处：《中华胰腺病杂志》2015年第1期22-25,共4页Chinese Journal of Pancreatology

基　　金：江苏省自然基金（BK2011487）;镇江市社会发展项目（SH2013026）;江苏大学大学生科研立项资助项目（12A209）

摘　　要：目的探讨缺氧诱导因子-1α 螯合四氧化三铁（HIF-1α @Fe3O4）纳米颗粒标记胰腺癌干细胞的可行性，并观察胰腺癌干细胞被标记后的增殖及凋亡变化。方法采用无血清培养法培养胰腺癌细胞株PANCl干细胞，将获得的干细胞与5、15、45、135μg/ml的HIF-lα @Fe304纳米颗粒共孵育24h，采用普鲁士蓝染色法观察胰腺癌干细胞的标记率，采用四甲基偶氮唑蓝比色法检测胰腺癌干细胞的增殖活性，采用流式细胞仪检测胰腺癌干细胞的凋亡。结果HIF-1α @Fe3O4纳米颗粒与胰腺癌干细胞共培养24h后的胞质内可见不同程度的蓝色铁染颗粒，且随着HIF-1α @Fe3O4浓度的增加而增加，浓度为45μg/m1时标记率达100％。未标记的干细胞及5、15、45、135μg/mlHIF-1α @Fe3O4标记的胰腺癌干细胞的细胞凋亡率分别为（3．76±0．96）％、（4．38±0．84）％、（4．36±1．22）％、（3．80±0．11）％、（4．78±0．98）％，差异无统计学意义（P〉0．05）；未标记的干细胞与各浓度纳米颗粒标记的干细胞细胞存活率的差异亦无统计学意义（P〉0．05）。结论HIF-1α @Fe3O4纳米颗粒可用于标记胰腺癌干细胞，且不影响胰腺癌干细胞的增殖及凋亡。Objective To explore the feasibility of HIF-lα@ Fe304 nanoparticles tag of pancreatic cancer stem cells, and to investigate the proliferation and apoptosis rate of the labeled pancreatic cancer stem cells. Methods PANC1 pancreatic cancer stem cells were cultured in serum-free medium, and then the pancreatic cancer stem cells were co-cultured with different concentrations of HIF-1α@ Fe3 04 （5 ,15,45 , 135 μg/ml） for 24 h. Prussian blue staining method was used to determine the labeling efficiency. MTT method was used to analyze the proliferation of pancreatic cancer stem cells. Also, flow eytometry was used to evaluate apoptosis. Results Prussian blue staining showed various number of blue-stained iron particles in the cells after co-culture of HIF-lα@ Fe30a nanoparticles and pancreatic cancer stem cells. And the number of blue- stained iron particles increased with the concentration of HIF-lα@ Fe304 , and the labeled rate reached 100% with the concentration of 45 μg/ml or above. The apoptosis rates of stem cells of 5, 15, 45, 135 μg/ml HIF- la@Fe304 tag and stem cells of without tag were （4.38±0.84）%, （4.36±1.22）%, （3.8±0.11）%, （4.78 ± 0.98 ）%, （3.76 ± 0.96 ）%, and the difference between the two groups was not statistically significant （P 〉0.05 ）; and the survival rates between the two groups was not statistically significant （ P 〉 0.05 ） , either. Conclusions HIF - 1 α @ Fe304 nanoparticles can be used for tagging pancreatic cancerstem cells, and they do not affect the proliferation and apoptosis of pancreatic cancer stem cells.

关 键 词：胰腺肿瘤 缺氧诱导因子-lα 四氧化三铁 干细胞 细胞增殖 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]