肝衰竭大鼠胃动力的改变及其机制的初步探讨  

Characters of antral motility in rats with liver failure

作　　者：朱金照[1] 王艳丽[1] 黎环[1] 刘晓燕[1] 熊伟[1] 吴旭玮[1] 

机构地区：[1]福建医科大学孟超肝胆医院肝病消化内科,福建福州350025

出　　处：《临床消化病杂志》2015年第1期9-12,共4页Chinese Journal of Clinical Gastroenterology

基　　金：福州市科技局科技计划项目(No:2011-S-69-3)

摘　　要：[目的]观察肝衰竭大鼠胃动力的变化并探讨其初步机制。[方法]40只Wistar大鼠随机分为肝衰竭模型组和对照组,采用葡聚糖蓝-2000为标记物观察大鼠胃排空的变化,应用乙酰胆碱酯酶(AchE)和NADPH-d组织化学染色(NOS)及肌间神经丛全层铺片技术,观察肝衰竭大鼠胃窦肌间神经丛胆碱能和氮能神经的变化,免疫组化染色观察胃窦C-kit阳性Cajal间质细胞的变化,并进行定量分析。[结果]与对照组比较,模型组大鼠胃排空明显减弱,胃窦肌间神经丛胆碱能阳性神经元数量减少、神经纤维变细、分布较稀疏,2组比较差异有统计学意义(P<0.01);模型组氮能神经阳性神经元数量及神经纤维分布明显高于对照组(P<0.01);胃窦C-kit阳性Cajal间质细胞明显少于对照组(P<0.01)。[结论]肝衰竭大鼠胃动力明显减退,其机制与胃窦肌间神经丛胆碱能神经分布减少、Cajal间质细胞减少及氮能神经分布增加有关。[Objective]To observe the changes of gastric empty and its mechanisms in rats with liver failure.[Methods]Forty Wistar rats were randomly divided into control group and liver failure model group,the gastric empty of rats were assayed by Dextran blue-2000 as a indicator;the cholinergic and nitrergic nerves in antrum myoenteric plexus were observed with acetylcholinesterase histochemical and NADPH-d histochemical staining,immunohistochemical staining was used to detected the content of C-kit positive ICC in antrum of rats,meanwhile analysed by computer.[Results]Comparing with control group,in model group,the gastric empty of rats was markedly delayed（P〈0.01）,both the distributions of myenteric cholinergic nerve and the content of C-kit positive ICC in antrum were significantly decreased（P〈0.01）,the distributions of myenteric nitrergic nerve were significantly increased（P〈0.01）.[Conclusion]The gastric empty in rats with liver failure was markedly delayed,which may be induced by the changes of cholinergic nerve and nitrergic nerve and ICC.

关 键 词：肝功能衰竭 胆碱能神经 氮能神经 CAJAL间质细胞 胃动力 

分 类 号：R575.3[医药卫生—消化系统]