LIN28基因对人胰腺癌细胞PANC1和SW1990增殖和迁移的影响  

Effects of LIN28 Gene on Proliferation and Migration of Human Pancreatic Cancer Cells PANC1 and SW1990

作　　者：卞石惠 李杰[1] 龚爱华[2] 陈晖[1] 蒋小猛[1] 张尤历[1] 徐岷[1] 

机构地区：[1]江苏大学附属医院,镇江212000 [2]江苏大学,镇江212000

出　　处：《中国细胞生物学学报》2015年第2期178-184,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81472333);江苏省自然科学基金(批准号:BK2013247)资助的课题~~

摘　　要：该文目的为研究LIN28基因对人胰腺癌细胞增殖和迁移的影响,揭示其在胰腺癌发生发展中的作用。实验发现,胰腺癌细胞中LIN28的表达模式与细胞增殖和迁移能力有关,将干扰质粒LIN28 sh RNA转染入LIN28高表达的PANC1细胞,将过表达载体3×Flag-LIN28转染入低表达的SW1990细胞中,采用RT-PCR和Western blot实验验证基因沉默或过表达的效果,并运用CCK-8法分析细胞的增殖能力并绘制细胞生长曲线,Transwell实验检测细胞迁移率。结果显示,下调LIN28后,PANC1细胞中LIN28基因的表达量明显降低,细胞增殖减慢,迁移减少;而上调LIN28后,SW1990细胞中LIN28基因的表达量明显增高,细胞增殖加快,迁移增加。由此说明,沉默LIN28基因可以抑制PANC1细胞增殖和迁移,过表达LIN28基因可以促进SW1990细胞增殖和迁移,这为阐明LIN28在胰腺癌演化过程中的机制奠定了实验基础。The paper aimed at investigating the effects of LIN28 expression in proliferation and migration of pancreatic cancer cells. The preliminary experiment results showed that the abilities of cell proliferation and migration of pancreatic cancer cells associated with the expression pattern of LIN28 gene. Then the specific sh RNA against LIN28 and the expressing vector 3×Flag-LIN28 were transiently transfected into the pancreatic cancer cells PANC1 and SW1990, respectively. The results of RT-PCR and Western blot demonstrated that the expression of LIN28 was efficiently inhibited or enhanced at both m RNA and protein levels（P〈0.05）. CCK-8 and transwell assays revealed that LIN28 depletion decreased cell proliferation and migration rates on PANC1 cells（P〈0.05）, and conversely LIN28 overexpression increased cell proliferation and migration rates on SW1990（P〈0.05）. Taken together, our results suggested that the expression level of LIN28 related to the activities of proliferation and migration of pancreatic cancer cells. This study will be an experimental support for clarifying the regulation role of LIN28 in pancreatic cancer evolution.

关 键 词：LIN28 胰腺癌 细胞增殖 细胞迁移 

分 类 号：R735.9[医药卫生—肿瘤]