p27在荔枝核总黄酮抑制人肝星状细胞LX2增殖过程中的表达及其意义  

Total flavonoids of litchi suppress proliferation of LX2 human hepatic stellate cells by upregulating p27 expression

作　　者：徐伶俐[1] 罗伟生[1] 谭宁[2] 徐庆[2] 徐宾[2] 诸葛福艳[2] 

机构地区：[1]桂林医学院附属医院消化内科,广西壮族自治区桂林市541004 [2]桂林医学院科学实验中心,广西壮族自治区桂林市541004

出　　处：《世界华人消化杂志》2015年第4期539-546,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81360530~~

摘　　要：目的:研究荔枝核总黄酮(total flavonoids of litchi,TFL)对人肝星状细胞LX2增殖的影响及其相关作用机制.方法:使用不同浓度(7.8125、15.6250、31.2500、62.5000、125.0000μg/m L)TFL处理LX2.采用CCK-8法检测细胞的增殖活力,通过流式细胞仪分析各组细胞周期的分布,使用RT-PCR和Western blot技术测定细胞中p27基因m RNA和蛋白的表达.结果:TFL作用48、72 h可抑制LX2细胞增殖,且随着时间延长,抑制效果更加明显.T F L处理72 h后可测得L X2细胞被阻滞在S期,且明显上调p27基因的m RNA和蛋白的表达.结论:TFL抑制人肝星状细胞增殖并将其阻滞在S期,该作用机制可能与p27表达上调相关.AIM： To investigate the effect of total flavonoids of litchi（TFL） on the proliferation of LX2 human hepatic stellate cells and to explore the underlying mechanism. METHODS： LX2 cells treated with different concentrations（7.8125, 15.6250, 31.2500, 62.5000, and 125.0000 μg/m L） of TFL were examined for cell growth inhibition using CCK-8. Flow cytometry was used to analyze the changes in cell cycle distribution of LX2 cells. The expression of p27 m RNA and protein in LX2 cells was determined by real-time quantitative PCR and Western blot, respectively. RESULTS： Exposure to TFL caused significant dose- and time-dependent inhibition of LX2 cell proliferation. TFL induced S-phase cell cycle arrest as shown by flow cytometric analysis. In addition, expression of p27 m RNA and protein in LX2 cells was upregulated in the treatment groups. CONCLUSION： TFL treatment inhibits LX2 cell proliferation and arrests cells at S phase, and the mechanism may be associated with the upregulation of p27 expression.

关 键 词：荔枝核总黄酮 人肝星状细胞 增殖 S期 P27 

分 类 号：R575.2[医药卫生—消化系统]