大肠癌、大肠腺瘤组织中Caspase3、P53及Ki-67表达的变化  

Expression of Caspase3,P53 and Ki-67 in colon cancer and colon adenoma

作　　者：毕大明 董艳玲[1] 吴金朋[1] 王雪宁[1] 胡二斌 郑建国[1] 刘娜娜[1] 

机构地区：[1]廊坊市第四人民医院病理室,河北省廊坊市065700

出　　处：《世界华人消化杂志》2015年第3期510-514,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨Caspase3在大肠癌中的表达及其与P53及Ki-67的相关性.方法:收集大肠癌、腺瘤组织标本各100例,免疫组织化学SP染色方法检测Caspase3、P53与Ki-67的阳性表达.相关性采用Spearman相关性分析.结果:Caspase3、P53和Ki-67的表达在不同年龄、性别、分化程度、浸润程度方面的大肠癌组织中差异没有统计学意义(P>0.05),但是其阳性表达率在淋巴结转移组(18.18%、31.82%、90.91%)与无淋巴结转移组(52.56%、89.74%、61.54%),Duke's分期中A-B期(52.56%、89.74%、61.54%)和C-D期(18.18%、31.82%、90.91%)中均存在明显差异(P<0.05).大肠癌组织中C a s p a s e 3、P 5 3以及K i-6 7阳性率分别为45.00%、77.00%、68.00%,大肠腺瘤组织中Caspase3、P53以及Ki-67阳性率分别为83.00%、49.00%、26.00%,差异均具有统计学意义(P<0.05).结论:Caspase3、P53与Ki-67均参与了大肠癌的发展过程,共同作用于大肠癌细胞,有助于判断其侵袭能力、转移和预后情况.AIM： To explore the expression of Caspase3, P53 and Ki-67 in colon cancer and colon adenoma. METHODS： The expression of Caspase3, P53 and Ki-67 in 100 colon cancer tissues and 100 colon adenoma tissues was detected by immunohistochemistry. Correlations between Caspase3, P53 and Ki-67 expression and between their expression and clincopatholgical parameters were investigated using Spearman correlation analysis.RESULTS： The expression of Caspase3, P53 and Ki-67 had no significant correlation with age, sex, tumor differentiation or infiltration（P〉0.05）. The positive expression rates of Caspase3, P53 and Ki-67 differed significantly between the lymph node metastasis group（18.18%, 31.82%, and 90.91%, respectively） and non-lymph node metastasis group（52.56%, 89.74%, and 61.54%, respectively） and between Duke＇s stage A-B（52.56%, 89.74%, and 61.54%, respectively） and C-D（18.18%, 31.82%, and 90.91%, respectively）（P〈0.05）. The positive expression rates of Caspase3, P53 and Ki-67 were 45.00%, 77.00% and 68.00%, respectively, in colon cancer tissues and 83.00%, 49.00% and 26.00%, respectively, in adenoma tissues（P〈0.01）. The expression of P53 was positively correlated with that of Caspase3（r = 0.315, P〈0.01）, but negatively correlated with that of Ki-67（r =-0.302, P〈0.01）. CONCLUSION： Caspase3, P53 and Ki-67 are all involved in the development and progression of colon cancer, and combined detection of their expression can help determine the invasion, metastasis and prognosis of this malignancy.

关 键 词：CASPASE3 免疫组织化学 P53 Ki-67 大肠癌 大肠腺瘤 

分 类 号：R735.34[医药卫生—肿瘤]