胃幽门螺杆菌采用不同方法检测结果比较  

Comparison of different methods for detecting Helicobacter pylori

作　　者：汪浩 邹文静 

机构地区：[1]苏州市九龙医院检验科,江苏省苏州市215021

出　　处：《世界华人消化杂志》2015年第3期525-529,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨几种不同检测方法对胃幽门螺杆菌(Helicobacter pylori,H.pylori)感染诊断的价值.方法:以13C尿素呼气试验(13C urea breath test13CUBT)作为"金标准",对由于消化系症状来苏州市九龙医院就诊的209例患者分别采用胶体金法(金标法)、酶联免疫试验(ELISA)、13CUBT、免疫印迹法(immunobiotting test,IBT)、病理组织切片进行检测计算各检测方法诊断H.pylori感染的阳性率、阴性率、灵敏度、准确度、Kappa值等性能指标.结果:金标法与13C UBT检出率相比差异具有统计学意义(χ2=5.961,P=0.015),ELISA与13C U B T检出率相比差异无统计学意义(χ2=2.636,P=0.104),IBT与13C UBT检出率相比差异无统计学意义(χ2=2.105,P=0.147),病理组织切片与13C UBT检出率相比差异无统计学意义(χ2=1.171,P=0.279).以13C UBT作为标准,金标法准确度为77.03%,ELISA为74.64%,I B T为82.78%,病理组织切片为87.56%;金标法Kappa值为0.424,ELISA为0.437,IBT为0.659,病理组织切片为0.752.结论:病理组织切片与13C UBT具有高度一致性,可以作为H.pylori感染筛查的方法之一.AIM：To explore the clinical value of different methods for detecting Helicobacter pylori（H.pylori）.METHODS：Using （13）C urea breath test（UBT）as the ＂gold standard＂,209 patients with gastrointestinal symptoms treated at our hospital underwent H.pylori detection by colloidal gold method,enzyme linked immunosorbent assay,UBT,immunobiotting test（IBT） and pathological biopsy.The detection rate of H.pylori infection,sensitivity,accuracy,Kappa value and other performance indicators were calculated and compared.RESULTS：The detection rate differed significantly between colloidal gold method and （13）C UBT（X2= 5.961,P = 0.015）,but showed no significant difference between ELISA and（13）C UBT（X2= 2.636,P = 0.104）,between IBT and （13）C UBT（X2 = 2.105,P = 0.147）,or between pathological biopsy and （13）C UBT（X2 =1.171,P =0.279）.Using （13）C UBT as the gold standard,the accuracy was 77.03%for colloidal gold method,74.64%for ELISA,82.78%for IBT,and 87.56%for pathological biopsy;the Kappa value was0.424 for colloidal gold method,0.437 for ELISA,0.659 for IBT,and 0.752 for pathological biopsy.CONCLUSION：Histopathology and （13）C UBT have a high degree of consistency and can be used as preferred methods for screening H.pylori infection.

关 键 词：胃幽门螺杆菌 病理组织切片 13C尿素呼气试验 诊断 

分 类 号：R573[医药卫生—消化系统]