Six1在宫颈癌组织中的表达及意义  

Expression and significance of Six1 in cervical cancer

作　　者：王彤[1] 朴龙镇[1] 崔雪莲[2] 杨洋[2] 南云泽[1] 

机构地区：[1]延边大学附属医院妇科,133000 [2]延边大学医学院病理学教研室

出　　处：《天津医药》2015年第3期249-251,共3页Tianjin Medical Journal

基　　金：国家自然科学基金(61371067);吉林省科技厅中青年科研创新团队领军人才项目(20130521017JH)

摘　　要：目的探讨Six1基因在宫颈癌组织中的表达情况及其临床意义。方法免疫组织化学SP法检测123例宫颈癌组织,49例宫颈上皮内瘤变(CIN)组织及32例正常宫颈组织中Six1蛋白的表达。体外培养人宫颈癌He La细胞,免疫荧光染色观察Six1蛋白在细胞中的定位。结果宫颈癌组织Six1蛋白阳性表达率(72.3%)高于CIN(28.6%)及正常宫颈组织(15.6%),χ2分别为13.118,10.058(均P<0.01)。不同肿瘤大小,淋巴结及血道转移情况的宫颈癌组织中Six1蛋白阳性表达率差异有统计学意义(均P<0.01)。Six1蛋白在He La细胞中主要定位于细胞浆和核仁。结论 Six1与宫颈癌的发生发展密切相关,可能成为宫颈癌的侵袭与转移的分子标志物。Objective To investigate the clinical significance of protein expression of Six1 in cervical cancer. Meth-ods The immunohistochemical （IHC） staining was applied to detect the expression of Six1 protein in normal cervical tis-sues （n=32）, cervical intraepithelial neoplasia （CIN） tissues （n=49） and cervical cancer tissues （n=123）. The localization of Six1 protein was detected in vitro cultured HeLa cells using immunofluorescence （IF） staining. Results The positive rate of Six1was significantly higher in cervical cancer （72.3%） than that of CIN tissues （28.6%） and normal cervical tissues （15.6%,χ^2=13.118 and 10.058 respectively, P〈0.01）. There were significance differences in expression levels of Six1 protein be-tween different tumor sizes and metastasis of cervical cancer （P 〈0.01）. The Six1 protein showed positive signals in cyto-plasm and nucleoli in HeLa cells. Conclusion Six1 expression is associated with cervical cancer, which may be a potential biomarker for invasion and metastasis of cervical cancer.

关 键 词：宫颈肿瘤 基因 同源盒 Six1 免疫组织化学 肿瘤浸润 肿瘤转移 HELA细胞 

分 类 号：R737.33[医药卫生—肿瘤]