COX-2与Survivin蛋白在人成釉细胞瘤中的表达及意义  

The expression of COX-2 and Survivin in ameloblastoma and its clinical significance

作　　者：李乐[1] 张兴乐[1] 王鹏[1] 刘雨[2] 

机构地区：[1]承德医学院附属医院口腔科,067000 [2]承德医学院附属医院手术部,067000

出　　处：《天津医药》2015年第3期270-273,共4页Tianjin Medical Journal

基　　金：河北省科技支撑计划项目(201121054)

摘　　要：目的研究环氧化合酶-2(COX-2)和生存素(Survivin)在人成釉细胞瘤(AB)中的表达情况。方法收集60例AB患者的肿瘤组织和正常口腔黏膜组织。60例AB患者中原发40例,复发20例;组织学分型:棘皮瘤型12例,滤泡型18例,丛状型18例,基底细胞型6例,促结缔组织增生型6例。采用免疫组化法和蛋白质免疫印迹法检测AB和正常口腔黏膜中COX-2和Survivin的表达,比较不同组织及分型的AB表达情况。结果 AB中COX-2蛋白的阳性表达率(82.7%)高于正常口腔黏膜(10.0%),Survivin表达阳性率(83.3%)高于正常口腔黏膜(16.7%)。AB中COX-2蛋白表达水平为0.781±0.142,高于正常口腔黏膜组织的0.179±0.056;Survivin蛋白表达水平为0.447±0.139,高于正常口腔黏膜组织的0.072±0.017,差异均有统计学意义。COX-2与Survivin的表达呈正相关(r=0.778,P<0.05)。不同组织学分型AB中的COX-2和Survivin表达阳性率差异无统计学意义。结论 COX-2、Survivin在AB中高表达,可能与AB的发生发展有关。Objective To investigate the expression of cyclooxygenase-2（COX-2）and Survivin in ameloblastoma （AB） tissues. Methods A total of 60 AB samples （primary AB 40 cases, recurrent AB 20 cases） and 60 normal oral mucosas were collected from the Affiliated Hospital of Chengde Medical College. The AB samples included 12 acanthomatous types, 18 follicular patterns, 18 plexiform patterns, 6 basal cell ameloblastomas and 6 desmoplastic ameloblastomas. The expres-sion levels of COX-2 and Survivin were detected by immunohistochemistry and Western blot methods in AB and normal oral mucosa. The expressions of COX-2 and Survivin were compared between different types and tissues of AB. Results The positive rate of COX-2 was significantly higher in AB （82.7%） than that in normal oral mucosa （10.0%）. The positive rate of Survivin was significantly higher in AB （83.3%） than that in normal oral mucosa tissues （16.7%）. The expression of COX-2 （0.781±0.142） was higher in AB than that of normal oral mucosa tissues （0.179±0.056）. The expression of Survivin （0.447± 0.139） was significantly higher in AB than those in normal oral mucosa tissues （0.072±0.017）. There was a positive correla-tion between the expression of COX-2 and Survivin （r=0.778,P〈0.05）. There were no significant differences in the positive expressions of COX-2 and Survivin between different types of AB （P〉0.05）. Conclusion COX-2 and Survivin were over-expressed in AB, which may be involved in the occurrence and development of AB.

关 键 词：成釉细胞瘤 环氧化合酶-2 生存素 免疫组织化学 印迹法 蛋白质 

分 类 号：R739.8[医药卫生—肿瘤]