XAV939抑制人肝癌HepG2细胞血管生成拟态的形成  

XAV939 inhibits vasculogenic mimicry formation in human hepatoma HepG2 cells

作　　者：汤晓颖[1] 庞林宾[2] 宋立文[3] 王洪林[1] 

机构地区：[1]重庆医科大学附属第一医院肝胆外科,重庆400016 [2]德州市人民医院普外科,山东德州253000 [3]重庆医科大学附属大学城医院肝胆外科,重庆401331

出　　处：《基础医学与临床》2015年第3期345-349,共5页Basic and Clinical Medicine

基　　金：重庆市科技攻关项目(9902200426-0001)

摘　　要：目的初步研究XAV939对人肝癌Hep G2细胞血管生成拟态形成的影响及其可能的机制。方法实验分为对照组和实验组(0.5和1μmol/L XAV939分别处理Hep G2细胞48 h);体外成管实验检测各组细胞形成血管拟态的能力,RT-PCR检测ZEB1及MMP-7 mRNA的表达,Western blot检测β-catenin、ZEB1和MMP-7蛋白的表达。结果对照组与实验组形成管状结构数目分别为9.67±0.70,5.67±0.64(0.5μmol/L)和2.27±0.81(1μmol/L),体外形成管道结构的数目减少(P<0.01);实验组ZEB1及MMP-7 mRNA表达和ZEB1、MMP-7及β-catenin蛋白表达均较对照组减少(P<0.05)。结论 XAV939能有效抑制人肝癌Hep G2细胞血管生成拟态的形成。Objective To investigate the impact of XAV939 on human hepatoma Hep G2 cells vascular mimicry and its possible mechanism. Methods Hep G2 cells were divided into control group and XAV939 treatment groups with dose of 0. 5 and 1 μmol / L; Tube formation of tumor cells experiment was performed to detect the forming tubes ability of each group,RT-PCR was used to detect expressed ZEB1 and MMP-7 mRNA levels,Western blot was used to detect expression of β-catenin,ZEB1 and MMP-7 on protein levels. Results The numbers of vascular mimicry in the control group and the experimental group were as followe. In the control group： 9. 67 ± 0. 70,0. 5 μmol / L group： 5. 67 ± 0. 64,1 μmol / L group： 2. 27 ± 0. 81,which means the number of pipeline structure formation reducing in vitro（ P 0. 01）. Experimental group compared with the control group,ZEB1 and MMP-7 mRNA expression reduced; ZEB1,MMP-7 and β-catenin protein expression decreased（ P 0. 05）. Conclusions XAV939 can effectively inhibit the vasculogenic mimicry formation in human hepatoma Hep G2 cells.

关 键 词：XAV939 HEPG2 WNT/Β-CATENIN 血管生成拟态 

分 类 号：R735.7[医药卫生—肿瘤]