拔牙创愈合对邻近微种植体骨界面生长因子影响的研究  

Influence of Healing Process of Extraction on Related Growth Factors in Microscrew-bone Interface of Implanted Titanium Microscrews Near the Extraction Wounds

作　　者：孙吉成[1] 胡赟[1] 郑雷蕾[1] 赵志河[2] 唐甜[2] 徐小淋 谢兵伍 

机构地区：[1]重庆医科大学口腔医学院口腔疾病与生物医学及重庆市重点实验室,重庆401147 [2]四川大学华西口腔医院正畸科,成都610041

出　　处：《四川大学学报（医学版）》2015年第2期222-227,共6页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金项目(No.81000463;No.81470772);重庆市自然科学基金(一般)项目(No.cstc2012jja10053);重庆市医学科研项目(No.20141013;No.2013-2-068);重庆市医学科研计划项目(No.2012-2-126)资助

摘　　要：目的通过检测模型犬邻近拔牙创微种植体骨界面与骨重建密切相关的生长因子——转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α),骨钙素(OC)和核心结合因子基因(Cbfa1)等的表达,探讨拔牙创愈合对邻近微种植体骨界面生长因子的影响。方法 12只18月龄健康雄性比格犬,采取自身对照,建立邻近拔牙创植入微种植体实验动物模型(实验组),以非拔牙处植入微种植体为对照(对照组),分别于第1、3、8、12周依次处死,获得各带微种植体的组织块,采用免疫组织化学和原位杂交法,检测TGF-β、TNF-α、OC蛋白和Cbfa1基因表达,并对其表达结果进行半定量分析。结果实验组TGF-β、TNF-α蛋白以及Cbfa1基因表达在各检测时段均强于对照组,与对照组比较,1周时TNF-α的表达,3周时TGF-β、TNF-α的表达差异均具有统计学意义(P<0.05),8周时Cbfa1基因的表达差异具有统计学意义(P<0.05);OC蛋白的表达两组均随着术后时间的延长表达增强,术后8周达高峰,术后3周时实验组表达弱于对照组,差异有统计学意义(P<0.05)。结论拔牙创邻近微种植体植入后早期炎症反应较重,但持续时间不长。骨界面骨改建进程随着愈合时间的增加持续活跃,过长的愈合时间会使骨形成相关因子表达削弱,在整个过程中种植体能保持稳定。Objective To detect the expression of growth factors relating to bone reconstructions in microscrew-bone interface of implanted titanium microscrews near the extraction wounds,and to determine the influence of healing process on the growth factors.Methods Eight male Beagle dogs（age 18months）were divided into experimental and control groups.Titanium microscrews were implanted near an extraction wound in the dogs in the experimental group,while the controls had implantation without extraction wounds.The dogs were sacrificed at1,3,8,12 weeks,respectively.Immunohistochemistry and in situ hybridization sections were performed to detect the expression of TGF-β,TNF-α,osteocalcin（OC）protein and Cbfa1 mRNA.Results The experimental group had higher levels of expression of TGF-β,TNF-αprotein and Cbfa1 mRNA than the controls,with significant differences appearing at week one for TNF-α（P〈0.05）,week three for TGF-βand TNF-α（P〈0.05）,and week eight for Cbfa1mRNA（P〈0.05）.The expression of OC increased over time and reached peak at week eight（P〈0.05）.Conclusion Microscrews implanted near extraction wounds can maintain stability.Severe inflammation occurs in the early stage of healing,but does not sustain.Bone remodeling remains active over the healing process.But prolonged healing phase without force loading could weaken the remodeling.

关 键 词：拔牙创 微种植体 转化生长因子-β 肿瘤坏死因子-α 骨钙素 CBFA1 

分 类 号：R782.11[医药卫生—口腔医学]