CYP2C19、ALDH2和MTHFR基因多态性与冠心病的相关性研究  

The Correlation Between the Polymorphism of P4502C19,ALDH2 and MTHFR with Coronary Heart Disease

作　　者：张冬青[1] 王海滨[1] 刘淼[1] 赵娇[1] 

机构地区：[1]解放军总医院第一附属医院检验科,北京100048

出　　处：《标记免疫分析与临床》2015年第3期207-210,214,共5页Labeled Immunoassays and Clinical Medicine

基　　金：国家高技术研究发展计划(863计划)子课题(2014AA022304)

摘　　要：目的探讨细胞色素P450 2C19(cytochrome P450 2C19,CYP2C19)、乙醛脱氢酶2(acetaldehyde dehydrogenase 2,ALDH2)和亚甲基四氢叶酸还原酶(methylene tetrahydrofolate reductase,MTHFR)基因多态性与冠心病的相关性。方法分别采用DNA微阵列芯片技术和PCR-芯片杂交方法,对187例冠心病患者和166名健康体检者的CYP2C19、ALDH2和MTHFR位点进行多态性分析。结果 CYP2C19*2/*2、ALDH2 AA和MTHFR TT基因型及频率在冠心病组分别为8.9%、12.3%和34.9%,在对照组分别为3.6%、1.8%和4.1%,两组比较,差异均具有统计学意义(P<0.05);Logistic回归分析发现ALDH2和MTHFR基因多态性是冠心病发生的重要危险因素,ALDH2 GA和AA基因型:OR值=2.09,MTHFR CT和TT基因型:OR值=2.11。结论 ALDH2和MTHFR基因多态性可增加冠心病的发生风险。Objective To investigate the correlation between the polymorphism of CYP2C19,ALDH2 and MTHFR with coronary heart disease. Methods The genotypes of the CYP2C19,ALDH2 and MTHFR were detected by DNA microarray and PCR-chip hybridization technology in 199 patients with coronary heart disease and 160 controls. Results The frequencies of CYP2C19 ＊ 2 / ＊ 2,ALDH2 AA and MTHFR TT were 8. 9%,12. 3% and 34. 9% in coronary heart disease patients,3. 6%,1. 8% and 4. 1% in controls,which showed significant differences between two groups（ P〈0. 05）. The ALDH2 and MTHFR polymorphism were the main risk factors for coronary heart disease. The OR value of genotype ALDH2 GA and AA was 2. 09,MTHFR CT and TT was 2. 11. Conclusion The genetic polymorphism of ALDH2 and MTHFR may increase the risk for coronary heart disease.

关 键 词：冠心病 CYP2C19 ALDH2 MTHFR 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]