食道癌患者唾液中miRNA-144基因标志物对食道癌早期诊断的临床价值分析  

Analysis on clinical value in the early diagnosis of esophageal cancer marker gene miRNA-144 in saliva in patients with esophageal cancer

作　　者：吴朔明[1] 吴多光[2] 潘引鹏[1] 邵仲凡[1] 

机构地区：[1]连云港市第一人民医院胸外科,江苏连云港222002 [2]中山大学附属孙逸仙纪念医院胸外科,广东广州510120

出　　处：《西藏医药》2015年第1期3-5,共3页Tibetan Medicine

摘　　要：目的研究食道癌患者唾液中mi RNA-144基因标志物对食道癌的早期诊断价值。方法采集2014年1月~2014年7月在我院收治的60例食道癌患者的唾液样本设为实验组,并随机选取50名中老年健康志愿者,采集其唾液标本设为对照组,两组样本在所采集年龄、性别比上无显著性差异（P≤0.05）,具有可比性。将两组样品离心后取上清和全唾液,用PCR仪检测mi RNA-144在唾液中的表达水平。结果唾液上清食道癌组和全唾液食道癌组mi RNA-144的表达水平均高于健康对照组（P〈0.05）,ROC曲线分析显示,唾液上清液中,当截断点mi RNA-144≥10时,敏感性为58.6%,特异性为91.0%,Az=0.875;全唾液中,在截断点mi RNA-144≥50时,敏感性为83.6%,特异性为87.0%,Az=0.764,无论是唾液上清还是全唾液,mi RNA-144都具有诊断价值。结论 mi RNA-144在食道癌患者唾液中存在高表达,可以作为早期诊断食道癌的基因标志物。Objective To investigate the mi RNA-144 gene markers in the saliva of patients with esophageal cancer and value for early diagnosis of esophageal cancer. Methods in our hospital 60 cases of esophageal cancer patients were collected from March 2011 to March 2014 in the saliva samples as the experimental group,And randomly selected 50 middle-aged healthy volunteers, collecting its saliva samples as the control group.Two samples were no significant difference in age and gender.The collection of saliva was put into a sterile test tube and the whole saliva and supernatants after centrifugattion.The contents of mi RNA-144 in the samples were detected by PCR.Results The levels of mi RNA-144 in both the whole saliva and saliva supernatant were significantly higher in esophageal cancer group than in the control group（P〈0.05）. The ROC curve analysis showed that,in saliva supernatant,the cut- off point was≥10 with a sensitivity of 58.6% and a specificity of 91.0% Az=0.875, In the whole saliva, the cut-off point of mi RNA-144 was≥ 100, with a sensitivity of 83.6% and A specificity of 87.0% Az=0.764.suggesting a moderate diagnostic value of mi RNA-144 in whole saliva and saliva supernatant.Conclusion mi RNA-144 was highly expressed in the saliva of patients with esophageal cancer,can be used as genetic markersfor early diagnosis of esophageal cancer.

关 键 词：miRNA-144 食道癌 早期诊断2 

分 类 号：R735.1[医药卫生—肿瘤]