A combined dynamic inhalation device designed for moxa-smoking toxicity testing  

作　　者：Lan Lei Chang Xiaorong Zhang Guoshan Shi Jia 

机构地区：[1]Acupuncture and Tuina School, Hunan University of Traditional Chinese Medicine [2]Institute of Neuroscience, Changsha Medical University

出　　处：《Journal of Traditional Chinese Medicine》2015年第1期90-98,共9页中医杂志（英文版）

基　　金：Supported by the Study of Warming Effect of Moxibustion and its Principle(the Major National Basic Research Program of China,No.2009CB522904);the Study of Safety of Moxibustion Products Based on High Performance Liquid Chromatography-Mass Spectrometry in Metabonomics Technology(Hunan Graduate Student Innovation Fund,No.CX2010B342)

摘　　要：OBJECTIVE： To design a combined dynamic inhalation device for testing the toxicity induced by moxa smoking. METHODS： The new apparatus （Patent No. 201120101911.5） includes air renewal and recycling systems, a gas generating device, a gas control unit, and a device to measure and control tem- perature and humidity. Sprague-dawley rats were tested for acute and sub-chronic toxicity after exposure to moxa-burning smoke.METHODS： The new apparatus （Patent No. 201120101911.5） includes air renewal and recycling systems, a gas generating device, a gas control unit, and a device to measure and control tem- perature and humidity. Sprague-dawley rats were tested for acute and sub-chronic toxicity after exposure to moxa-burning smoke.RESULTS： We found an LQ0 of 1.2× 10^4 mg/m^3 in the acute toxicity assays. In sub-chronic toxicity tests the organ coefficients studied showed no sig-nificant differences within rats groups of the same gender after treatment with moxa smoke or a month of recovery. However, mean gray degree of lung 70 heat shock protein （HSP70） was significantly elevated in the high dose group in comparison with the low dose group （P 〈 0.05）, mean gray degree, mean optical density, gross area of HSP70 in other organs and caspase-9 parameters showed no significant differences between groups.CONCLUSION： These results suggest that moxa smoke had no overt toxicity in rats. This work pro- vides evidence and reference for the design of dy- namic inhalation exposure systems.OBJECTIVE: To design a combined dynamic inhalation device for testing the toxicity induced by moxa smoking.METHODS: The new apparatus(Patent No.201120101911.5) includes air renewal and recycling systems, a gas generating device, a gas control unit, and a device to measure and control temperature and humidity. Sprague-dawley rats were tested for acute and sub-chronic toxicity after exposure to moxa-burning smoke.RESULTS: We found an LC50 of 1.2 × 104mg/m3 in the acute toxicity assays. In sub-chronic toxicity tests the organ coefficients studied showed no sig-nificant differences within rats groups of the same gender after treatment with moxa smoke or a month of recovery. However, mean gray degree of lung 70 heat shock protein(HSP70) was significantly elevated in the high dose group in comparison with the low dose group(P < 0.05); mean gray degree, mean optical density, gross area of HSP70 in other organs and caspase-9 parameters showed no significant differences between groups.CONCLUSION: These results suggest that moxa smoke had no overt toxicity in rats. This work provides evidence and reference for the design of dynamic inhalation exposure systems.

关 键 词：MOXIBUSTION INHALATION TOXICITY HSP70 heat-shock proteins 

分 类 号：R245.81[医药卫生—针灸推拿学]