2010-2013年广东省深圳市南山区腹泻患者沙门菌血清学和脉冲场凝胶电泳分析  

Serological analysis and pulsed-field gel electrophoresis typing on Salmonella isolated from patients with diarrhea in Nanshan district of Shenzhen,Guangdong,2010-2013

作　　者：鞠长燕[1] 潘伟光 黄锐敏[1] 王婷[1] 陈辉[1] 

机构地区：[1]深圳市南山区疾病预防控制中心,深圳南山518054 [2]深圳市第六人民医院,广东深圳518000

出　　处：《疾病监测》2015年第1期30-34,共5页Disease Surveillance

摘　　要：目的分析广东省深圳市南山区2010—2013年哨点医院腹泻患者分离的沙门菌血清型分布和脉冲场凝胶电泳（PFGE）带型。方法对1868份腹泻患者的粪便标本进行沙门菌分离、鉴定、血清学分型和脉冲场凝胶电泳。结果共分离到92株沙门菌，总分离率为4．93％。4-10岁年龄组腹泻病例的沙门菌分离率最高，达23．21％，1～3岁组次之，达8．26％，〈1岁组和〉11岁各年龄组分离率差异无统计学意义，介于1．45％～5．41％。性别和分离年份差异无统计学意义。92株沙门菌分布于19种血清型，鼠伤寒沙门菌最多，占38．04％，肠炎沙门菌次之，占31．52％，第3位是斯坦利沙门菌，占7．61％。61株沙门菌可以分为41种PFGE带型，每种带型最多有7株菌。鼠伤寒门菌产生17个PFGE带型，主要带型为T1型，肠炎沙门菌的带型为E1型。发现2起家庭聚集性的沙门菌感染，1起为罗森沙门菌感染导致的1对双胞胎幼儿发病，另1起为1对夫妇和1位老人在家庭聚餐后感染肠炎沙门菌。结论4—10岁年龄组腹泻病例沙门菌分离率最高，需高度重视该年龄组的卫生习惯和卫生状况；本地区腹泻患者沙门菌血清型以鼠伤寒沙门菌、肠炎沙门菌为主；PFGE分型及时能对传染病的预防和控制起到重要作用。Objective To understand the serological characteristics of Salmonella circulating in diarrhea patients in Nanshan district of Shenzhen, Guangdong province. Methods A total of 1868 stool samples were collected from local diarrhea patients from 2010 to 2013 to condcut Salmonella isolation, identification, serotyping and related pulsed-field gel electrophoresis. Results A total of 92 Salmonella strains were isolated, the isolation rate was 4. 93 %. The isolation rate was highest in age group 4 - 10 years （23.21%）, followed by age group 1 -3 years （8.26%）. There was no significant difference in isolation rate between age groups 〈 1 year （ 1.45 % ） and age group 〉 11 years （5.41% ）. There was no sex and year specific significant differences in isolation rate. The Salmonella strains were in 19 serogroups. S. Typhimurium was the major serogroup, accounting for 38.04% , followed by S. Enteritidis, accounting for 31.52%, and S. Stanley, accounting for 7.61%. Forty-one PFGE patterns were detected in 61 strains, at least 7 strains were in one PFGE pattern. S. Typhimurium strains had 17 PFGE patterns, T1 was the major pattern. E1 was the major PFGE pattern of S. Enteritidis strains. Two household clustering of Salmonella infection were detected, one was S. Rissen infection in a twins, and another one was S. Enteritidithe infection in a couple and their mother. Conclusion The Salmonella isolation rate was highest in age group 4-10 years, it is necessary to take measures to improve the lifestyle and health status of this group. S. Typhimurium and S. Enteritidis were the major serogroups circulating in this area. Timely PFGE typing can play a important role in the prevention and control of infectious diseases.

关 键 词：沙门菌 脉冲场凝胶电泳 血清分型 腹泻 

分 类 号：R574.62[医药卫生—消化系统]