雌二醇(E2)与塑化剂(DIOP)对褐牙鲆幼鱼组织损伤初步研究  

Preliminary Study of 17β-Estradiol And Dio- Ctylphthalate(Diop)on Tissue Injury of Juvenile Paralichthys Olivaceus

作　　者：刘志峰[1,2] 洪磊[2] 郝振林[1] 雷霁霖[2] 

机构地区：[1]大连海洋大学,辽宁大连116023 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《海洋湖沼通报》2015年第1期96-106,共11页Transactions of Oceanology and Limnology

基　　金：国家现代农业产业技术体系建设专项资金资助项目(nycytx-50);中央级公益性科研院所基本科研业务费专项资金(2010-cb-05);中国博士后基金(20100471497)资助

摘　　要：分别采用浓度0.1μmol/L、1μmol/L的雌二醇(E2)和塑化剂(邻苯二甲酸二异辛酯,DIOP)对褐牙鲆幼鱼进行连续5周的暴露实验,取鳃、肝、肾进行常规石蜡切片,HE染色,显微镜观察。实验结果显示,在2种试剂的暴露胁迫下,鱼体组织产生不同程度的损伤,随着试剂浓度的增加、暴露时间的延长而变化明显。研究表明,E2可以导致鳃小片弯曲变细,末端弯曲膨大,鳃小片断裂,细胞坏死、脱落;肝细胞排列紊乱,肝细胞肿胀,出现脂肪变性和空泡化;肾小管形态崩解,肾小管变细,肾小管管腔变大,肾小球结构遭到破坏。DIOP可以促使鳃小叶之间发生组织增生、粘连,鳃小叶末端弯曲膨大,出现非组织性空腔;肝细胞出现轻微的细胞脂肪变性和空泡化;肾小管形态遭到破坏,出现管细胞受损或不可见现象。The juvenile Paralichthys olivaceus was exposed to 0.1μMol/L,1μMol/L E2 and plasticizer（Dio-ctylphthalate,DIOP）for 5weeks.The gill,liver and kidney were selected to make paraffin sections,then stained by HE and observed by microscope.Experimental results showed that exposed to the two kinds of pollutants,the fish can produce tissue damage,and with the serious damage when increasing of concentration and the extension of time.Paraffin section showed that the gill lamella became bending and thin with intumescent terminal,and even became fractured with deciduous and necrosis cell in the group of E2.Disordered arrangement of liver cell,swollen liver cell,fatty degeneration and cavitation appeared and renal tubular form became disintegrated,which renal tubule becoming thinner,lumen of renal tubule becoming bigger and glomerular structure being destroyed in the group of E2.While in the group of plasticizer,intumescent and bend terminal of the Gill lamella,abnormal cavity appeared with and adhesion of the Gill lamella.In addition,slight fatty degeneration and cavitation also appeared,and renal tubular form was destroyed with damaged or invisible tubular cells.

关 键 词：E2 塑化剂 褐牙鲆 组织损伤 

分 类 号：S917.4[农业科学—水产科学]