化学合成面筋蛋白外啡肽B5对鸡淋巴细胞体外免疫活性的影响  

Effects of Chemically Synthesized Gluten Exorphin-B5 on Ymphocyte Immune Activity of Chicken in Vitro

作　　者：周业飞[1] 周梅仙[1] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,南京211171

出　　处：《西北农业学报》2015年第3期39-43,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：南京晓庄学院重点应用项目(2012NXY10)

摘　　要：面筋蛋白外啡肽B5是一种通过面筋蛋白的体外酶解所获得的阿片肽,具有一定的免疫学功能,有望成为一新型免疫增强剂。本研究通过化学合成方法制备,并将其加入鸡外周血单个核细胞中,以观察其对淋巴细胞的增殖效应和对IFN-γ和IL-4mRNA水平影响。结果显示,化学合成获得了高纯度的外啡肽B5(HPLC质量分数大于98.435 7%);外啡肽B5能显著促进鸡淋巴细胞的增殖,并能显著提高IFN-γ的mRNA水平(P<0.05),对IL-4mRNA无显著影响。Gluten exorphin B5（GE-B5）was a food-derived opioid peptide obtained by vitro enzymatic digestion of wheat gluten,and it had some immune function and maybe become a good candidate adjuvant for vaccine development.In this study,we chemically synthesized GE-B5 and added it to the nonvaccinated chicken peripheral blood mononuclear cells（PBMC）to observe the effects of proliferation on lymphocyte and.IFN-γmRNA levels.The results showed that GE-B5 of high purity had been obtained by synthesizing chemically（mass fraction of HPLC is higher than 98.435 7%）,GE-B5 could significantly increase cell proliferation and IL-4mRNA levels（P〈0.05）,but it had no effect on IL-4mRNA.

关 键 词：面筋蛋白 外啡肽B5 淋巴细胞 IFN-Γ 

分 类 号：TQ28[化学工程—有机化工]