HLA-DRB多态性与HBV感染的相关性研究  

The correlation between HLA-DRB polymorphism and HBV infection

作　　者：邢文斌 郭晓楠 徐慧宁 

机构地区：[1]大连市第六人民医院肝病科,大连市600031

出　　处：《中华实验和临床感染病杂志（电子版）》2015年第1期63-66,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：大连市卫生局科研基金项目(No.2012.-021)

摘　　要：目的通过对慢性乙型肝炎男性患者的配偶和子女HBV感染状况及其HLA-DRB多态性分析,揭示HLA-DRB与HBV感染的相关性。方法采用ELISA方法筛查HBV血清标志物,采用PCR/SSP技术扩增HBV感染者及易感人群HLA-DRB1不同位点等位基因片段,琼脂凝胶电泳分离基因片段,紫外透射反射分析仪判定HLA-DRB1型别。结果慢性乙型肝炎患者及HBV携带者携带HLA-DRB1*0201、0301基因显著高于健康对照组;HLA-DRB1*1301在健康对照组的携带率显著高于慢性乙型肝炎患者及病毒携带者;HLA-DRB1*0201、0301、0701和1301在感染后恢复组与健康对照组相比携带率差异无统计学意义;HLA-DRB1*0201、0301、0701和1301等位基因在肝炎组与携带组中差异无统计学意义;HLA-DRB1*0201、0301和0701在肝炎组携带率率较高,HLADRB1*1301在感染后恢复组携带率较高。结论 HLA-DRB1*0701、0301、0201与HBV慢性感染有关,HLA-DRB1*1301与HBV的清除有关。Objective To analyze the HBV infection status and their HLA-DRB polymorphism among male patients with chronic hepatitis B, meanwhile their spouses and children were collected, to identify the correlation between HLA-DRB and HBV. Methods The serum markers of hepatitis B were tested by ELISA, different HLA-DRB1 allelic fragments in the patients with HBV and their spouses and children were ampliifed by ampliifcation, and the gene fragments were separated by agar gel electrophoresis, UV transmittance reflectance analyzer determine the type of HLA-DRB1. Results The patients with chronic hepatitis B and HBV carriers carrying HLA-DRB1＊0201, 0301 genes were signiifcantly higher than those of healthy control group. The frequency of HLA-DRB1＊1301 allele in healthy control group were higher than those in HBV patients and carriers. There was no significant difference between the recovery group and healthy control group with HLA-DRB1＊0201, 0301, 0701 and 1301 alleles. The frequency of HLA-DRB1＊0201, 0301, 0701 and 1301 alleles between the patients with chronic hepatitis B and HBV carriers were no significant difference. The frequency of HLA-DRB1＊0201, 0301 and 0701 alleles in the hepatitis group was higher, and HLA-DRB1＊1301 allele in recovery group was higher. Conclusions HLA-DRB1＊0701, 0301 and 0201 alleles were related to HBV chronic infection, and HLA-DRB1＊1301 allele was related to the clearance of HBV.

关 键 词：HBV感染 肝炎 乙型 慢性 配偶和孩子 HLA-DRB1 等位基因 

分 类 号：R512.62[医药卫生—内科学]