大鼠脑出血后同侧海马区Slit2、 Nogo-A表达的相关研究  

Relationship study of the expressions of Slit2 and Nogo-A in rats with cerebral hemorrhage

作　　者：王风波[1] 王琼芬[1] 张弘[1] 李飞[1] 唐明薇[2] 

机构地区：[1]成都医学院第一附属医院康复医学科,成都610500 [2]成都医学院第一附属医院内分泌科,成都610500

出　　处：《中国医师杂志》2015年第2期195-197,共3页Journal of Chinese Physician

基　　金：2012年度四川省卫生厅科研课题资助项目（120476）

摘　　要：目的 观察大鼠脑出血后同侧海马区Slit2、Nogo-A的表达情况.方法 将64只成年SD大鼠按随机数字表法分为对照组（n=32）、模型组（n=32）.模型组采用Ⅶ型胶原酶制备诱导尾状核出血模型;对照组手术操作方法同模型组,注射同等剂量生理盐水.2组大鼠分别于术后24h、7、14及21 d时相点行出血侧海马区免疫组化Slit2、Nogo-A检测.结果 与对照组相比,模型组Slit2、Nogo-A阳性反应均明显增强（P＜0.01）,以7d最为显著.结论 脑出血可引起Slit2、Nogo-A的表达显著增强,两者呈正相关,可能对脑损伤后的修复有着重要的影响作用.Objective To investigate the expressions of Slit2 and Nogo-A in the ipsilateral hippocampus after cerebral hemorrhage in rats.Methods A total of 64 adult Sprague-Dawley （SD） rats was randomly divided into control and model groups.Collagenase Ⅶ was used to induce cerebral hemorrhage model.Immunohistochemistry was used to detect the expressions of Slit2 and Nogo-A in hippocampus at the cerebral hemorrhage side at the time points （24 h,7 d,14 d,and 21 d）.Results Compared to the control group,the expressions of Slit2 and Nogo-A were significantly enhanced in model group （P 〈0.01）,with the highest level at the 7 d.Conclutions Cerebral hemorrhage can significantly enhance expressions of Slit2 and Nogo-A with a positive correlation of Slit2 and Nogo-A,which might have an important effect on the recovery of brain injury.

关 键 词：脑出血/代谢/病理学 神经组织蛋白质类/代谢 髓磷脂蛋白质类/代谢 

分 类 号：R743.34[医药卫生—神经病学与精神病学]