人毛乳头细胞微囊诱导大鼠足垫毛囊形成模型的建立  

Model of hair follicle regeneration model induced by microencapsulated dermal papilla cells

作　　者：林常敏[1] 蔡湘娜[2] 蔡博治[3] 李宇[2] 黄铿[4] 

机构地区：[1]汕头大学医学院组织学与胚胎学教研室,广东汕头515041 [2]汕头大学医学院第一附属医院美容整形外科 [3]汕头大学医学院第一附属医院组织工程实验室 [4]汕头大学医学院第二附属医院急诊外科

出　　处：《中国美容整形外科杂志》2015年第3期186-189,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金面上项目

摘　　要：目的 建立可靠的、异种毛乳头诱导毛发形成的动物模型.方法 以局部注射方式将毛乳头细胞微囊移植至大鼠足垫.移植后第2周至10个月期间定期取材,观察毛囊结构形成的过程,免疫组化追踪毛囊形成过程K15（Keratinl5,角蛋白15）的时空变化.结果 移植后第10周,移植部位皮肤出现肉眼可见的毛发纤维.表皮干细胞标记物K15免疫组化染色显示,处于毛乳头细胞微囊上方的表皮干细胞逐渐向皮下组织迁移.结论 本研究完全排除了受体动物自身毛囊细胞的干扰,为研究毛囊发育过程中上皮细胞与真皮细胞间相互作用的调节机制提供了理想的动物模型.Objective To create a stable animal model for the xenotransplantation of human dermal papilla cells microcapsnles into rat footpad to induce the hair follicles formation and growth. Methods Cultured dermal pa- pilla ceils were encapsulated and then implanted into the rat footpads. After the xenotransplantation, observed them timely and studied the histomorphology by microscopy from the second week to the tenth month. K15 immunohisto- chemistry stain and analyzed the relationship between them. Results At the tenth week following transplantation, hair fibers were visible in the footpad. K15 positive cells were transmitting from basal layer to subcutaneous and ob- served near the dermal papilla cell microcapsules. Conclusion dermal papilla cell microcapstfies retain the capacity to initiate follicle regeneration and suitable for allograft or xenotransplatation. The animal model of dermal papilla cells microcapsule induces hair follicles regeneration in rat footpad exclude the effect of hair follicle epithelial cells. It is a stable model for study the interaction of epidermis and dermis during the formation of hair follicle.

关 键 词：毛囊 毛乳头 诱导 动物模型 足垫 

分 类 号：R-332[医药卫生]