AKT和GSK3β在人脑胶质瘤中的表达  

Correlation of prote in expression level of AKT and GSK3β with glioma

作　　者：廖宇钦[1] 丁建军[1] 高永中[1] 关弘[1] 孙艳花[1] 许美权[1] 伍建明[1] 陈盛强[2] 

机构地区：[1]深圳市第二人民医院,广东深圳518000 [2]广州医科大学第二附属医院,广州510260

出　　处：《解剖学研究》2015年第1期10-14,共5页Anatomy Research

基　　金：深圳市科技计划项目(No.201203059)

摘　　要：目的通过观察AKT和GSK3β蛋白在人脑胶质瘤中的表达变化,探讨AKT和GSK3β在脑胶质瘤发生中的关系及临床意义。方法应用免疫组织化学(IHC)SP法检测90例脑胶质瘤组织及15例正常脑组织中AKT和GSK3β蛋白的表达变化。结果正常脑组织与胶质瘤组织中AKT蛋白阳性率分别是30.00%与85.71%,两者差异有统计学意义(P<0.05);AKT蛋白在低级别胶质瘤和高级别胶质瘤中的阳性率分别是65.71%与98.18%,两者之间差异有统计学意义(P<0.05);GSK3β蛋白在低级别胶质瘤和高级别胶质瘤中的阳性率分别是48.57%与21.82%,两者之间差异有统计学意义(P<0.05)。结论胶质瘤组织中AKT蛋白表达明显增加,且随着胶质瘤恶性程度的增加而增加,胶质瘤组织中GSK3β蛋白表达明显降低,且随着胶质瘤恶性程度的增加而降低,提示AKT和GSK3β蛋白在胶质瘤发生中相互作用。Objective To investigate the correlation of protein expression level of AKT and GSK3βwith infiltration and metastasis in glioma.Methods The protein expression level of AKT and GSK3βwas detected by immunohistochemistry（IHC）with Strep tavidin-Peroxidase（SP） in 90 case of esophageal squamous cell carcinoma（ESCC） and 15 case of normal oesophagus tissues.Results The result showed that the positive expression rate of m TOR in carcinoma was higher than that in normal tissues（P〈0.05）;The expression of AKT was correlated with fibromembranous invasion（P〈0.05） and lymphnode memtastasis（P〈0.05）;The result showed that the positive expression rate of GSK3β in carcinoma was lower than that in normal tissues（P 0.05）;The expression of GSK3βwas correlated with fibromembranous invasion（P 0.05） and lymphnode memtastasis（P 0.05）Conclusion Immunohistochemical detection of AKT expression may carry clinical significance inevaluating the degree of differentiation,invasiveness,metastaticpotential and p rognosis of glioma

关 键 词：脑胶质瘤 丝氨酸-苏氨酸蛋白激酶 糖原合成酶激酶-3Β 

分 类 号：R739.41[医药卫生—肿瘤]