他米巴罗汀对牙龈卟啉单胞菌诱导的牙周炎模型固有免疫应答的影响  

Effect of tamibarotene on innate immune response in the model of Porphyromonas gingivalis induced periodontitis

作　　者：靳赢[1] 刘迪昕[1] 方絮[1] 高科北 林晓萍[1] 

机构地区：[1]中国医科大学附属盛京医院口腔科,沈阳110004

出　　处：《中华口腔医学杂志》2015年第3期146-150,共5页Chinese Journal of Stomatology

基　　金：辽宁省科学技术计划（2012225015）;沈阳市科学技术项目计划（F13-221-9-28）;高等学校博士学科点专项科研基金（20112104110013）

摘　　要：目的 探讨他米巴罗汀（tamibarotene,Am80）对牙周致病菌牙龈卟啉单胞菌（Porphyromonas gingivalis,Pg）W83诱导的牙周炎小鼠固有免疫应答的影响,为早期免疫调控牙周炎提供实验基础.方法 将25只小鼠按随机数字表法分为5组：正常对照组、PgW83口腔涂抹诱导的牙周炎组（牙周炎组）、10 μg/d Am80治疗组（低剂量治疗组）、50 μg/d Am80治疗组（中剂量治疗组）、100 μg/dl Am80治疗组（高剂量治疗组）,每组5只;解剖显微镜下观察并测量各组小鼠牙槽骨吸收的距离;采用酶联免疫吸附实验检测血清抗Pg特异性IgG的水平;实时定量PCR法检测牙龈组织、引流淋巴结及脾脏中白细胞介素（interleukin,IL）12和干扰素γmRNA的表达情况.计量资料比较采用单因素方差分析,LSD检验比较两组间差异,以双侧P＜0.05为差异有统计学意义.结果 中剂量[（121±10）％]和高剂量治疗组骨吸收率[（108±8）％]与牙周炎组[（133±10）％]相比差异均有统计学意义（P＜0.05）;低、中、高剂量治疗组小鼠血清抗Pg特异性IgG水平（分别为0.437±0.083、0.566±0.012及0.386±0.078）均显著低于牙周炎组（1.151±0.433） （P＜0.001）;实时定量PCR结果显示：Am80处理后,小鼠牙龈组织、淋巴结、脾脏中IL-12 mRNA相对表达水平分别降低至1.107±0.088、0.806±0.220、0.668±0.756,与牙周炎组相比差异均有统计学意义（P＜0.01）;干扰素γmRNA相对表达水平分别降至8.898±0.427、16.654±5.995、1.482±0.033,与牙周炎组相比差异均有统计学意义（P＜0.001）.结论 早期应用Am80可通过影响牙周炎小鼠固有免疫应答减轻小鼠牙周炎症程度,缓解牙槽骨吸收.Objective To investigate the effect of Am80 on innate immune response of Porphyromonas gingivalis（Pg） W83-induced periodontitis in mice.Methods Twenty-five mice were randomly divided into five groups,control group,PgW83-induced periodontitis group（periodontitis）,Am80 （10 μg/d） treatment group（low-dose group）,Am80（50 μg/d） treatment group（middle-dose group）,Am80 （100 μg/d） treatment group（high-dose group）.The distance of alveolar bone resorption in each mouse was observed and measured lby a dissecting microscope.Enzyme-linked immunosorbent assay（ELISA） was used to detect the level of serum anti-Pg specific IgG.The mRNA expression of interferon-γ（IFN-γ） and interleuking-12（IL-12） in gingival tissues,draining lymph node and spleen were detected bv real-time reverse transcriptase polymerase chain reaction（RT-PCR）.The measurement data were statistically analvzed.Results The resorption rate in Am80 group[（121 ± 10）%] and high-dose of Am80 group[（108±8）%] was significantly different with that in periodontitis mice[（133± 10）%]（P〈0.05）.The serum levels of anti-Pg specific IgG of the Am80 groups of different doses（0.437 ± 0.083,0.566 ± 0.012,and 0.386± 0.078） were significantly lower than that of the periodontitis group（l.151±0.433） （P〈0.001）.Real-time quantitative PCR assay showed that after Am80 treatment,the IL-12 mRNA levels in the gingival tissues,lymph nodes and spleen of mice were reduced to 1.107±0.088,0.806±0.220,and 0.668±0.756,which were all significantly different with those in periodontitis（P〈0.01）.Similarly,the relative expression of IFN-γ mRNA levels in gingival tissue,lymph nodes and spleen of mice were reduced to 8.898±0.427,16.654±5.995,and 1.482± 0.033,which were significantly different with periodontitis（P〈0.001）.Conclusions Am80 can reduce the extent of inflammation and alleviate alveolar bone resorption by modulating innate immune response.

关 键 词：牙周炎 牙槽骨丢失 他米巴罗汀 固有免疫应答 

分 类 号：R781.42[医药卫生—口腔医学]