小鼠老化淀粉样纤维在骨骼肌中的沉积  

Deposition of mouse senile amyloid fibrils in the skeletal muscles

作　　者：钱金泽[1] 霍佳[2] 钱俊乔[3] 李陈莉[4] 赵昱[4] 樋口京一 段惠军[1] 

机构地区：[1]河北医科大学 病理学教研室,石家庄050017 [2]河北医科大学附属第三医院骨病科,石家庄050017 [3]河北医科大学口腔医院,石家庄050017 [4]河北医科大学组织学与胚胎学教研室,石家庄050017 [5]日本信州大学医学部加龄生物学教研室,松本390862

出　　处：《解剖学杂志》2015年第1期31-34,共4页Chinese Journal of Anatomy

基　　金：第51批国家博士后基金面上资助(2012M510943);河北省青年自然科学基金(H2012206080);2012年河北医科大学科研骨干人才培育计划资助(201204213);国家自然科学基金青年基金项目(81300606);2012年河北省留学人员科技活动项目择优资助经费启动类项目

摘　　要：目的：探讨载脂蛋龄Ⅱ形成的小鼠老化淀粉样纤维是否可形成并沉积于骨骼肌中。方法：采用R1．P1-Apoa2c小鼠进行淀粉样变诱导实验，诱导实验进行2个月后，通过组织学、免疫组织化学、透射电镜及分子生物学检测骨骼肌中淀粉样纤维的沉积程度。结果：通过淀粉样变诱导实验2个月及4个月后，淀粉样纤维主要沉积于小鼠骨骼肌中的结缔组织及肌内膜内。结论：小鼠老化淀粉样纤维可在骨骼肌肌内膜及结缔组织中沉积，并为淀粉样变疾病的发生机制提供了新的理论依据。Objective： To explore if AAPOAⅡ amyloid fibrils could form and deposit in the musculoskeletal systems. Methods： AApoAⅡ amyloid fibrils were injected into R1. P1-Apoa2c mice to induce AAPOAⅡ systemic amyloidosis. Two months later, AAPOAⅡ amyloid deposits were observed in the skeletal muscles of amyloi＆affected mice, primarily in the blood vessels and in the interstitial tissues surrounding muscle fibers by histology, immunobistochemistry and transmission electron microscopy. Results： Amyloid deposits were found mainly in the blood vessels of the muscle tissues, but were also found in the connective tissues around muscle fibers （endomysium） in mice. Conclusion： These findings present that AAPOA Ⅱ amyloid fibrils could deposit in the skeletal muscles, which shed new light on the etiology of disorders involving amyloid.

关 键 词：小鼠老化淀粉样纤维 骨骼肌 免疫组织化学 电镜 

分 类 号：R363[医药卫生—病理学]