小鼠脑出血模型的行为学及海马神经元电生理变化研究  

Electrophysiology of hippocampal neurons and behavior characteristics in mouse models of intracerebral hemorrhage

作　　者：陈秉宇[1] 王震[1] 刘旭玲[2] 应优敏[3] 莫云长[2] 王均炉[2] 

机构地区：[1]浙江省人民医院输血科,杭州310014 [2]温州医科大学附属第一医院麻醉科,温州325000 [3]浙江大学药学院,杭州310014

出　　处：《中华神经医学杂志》2015年第3期254-258,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（81273926）;浙江省中医药科学研究基金（2014zB007）;浙江省中医药优秀青年人才基金（2014zQ005）

摘　　要：目的 探讨脑出血小鼠学习能力、脑水肿改变及海马神经元电生理特性的变化规律. 方法 选取80只雄性C57/BL6小鼠按随机数字表法分为脑出血组和对照组,其中脑出血组40只,对照组40只.脑出血组取20 μL尾动脉自体血,应用立体定位技术注入小鼠尾状核;对照组采用生理盐水同法注射.造模后1、3、5和7d对小鼠进行神经功能缺损评分评定,应用Morris水迷宫实验（定位航行实验、空间探索实验）观察小鼠的行为学表现,应用干湿重法检测脑组织含水量,应用全细胞膜片钳技术和计算机软件检测记录海马神经元电生理特性. 结果 与对照组相比,造模后1、3、5和7d脑出血组小鼠神经功能缺损评分明显增高,训练第5天脑出血组小鼠在站台所在象限停留时间比率明显降低,造模后1、3、5和7d脑出血组小鼠血肿周围脑组织含水量明显升高;脑出血组小鼠海马CA1区锥体细胞的静息膜电位和输入电阻明显降低,差异均有统计学意义（P＜0.05）. 结论 小鼠发生脑出血后海马依赖的空间学习记忆能力降低,海马神经元钾离子通道的通透性增强.Objective To establish mouse models of intracerebral hemorrhage using autologous arterial blood,to study the physiological property of hippocampal neurons,brain edema changes and learning ability in the mouse models after intracerebral hemorrhage.Methods Eighty male C57/BL6 mice were randomly divided into intracerebral hemorrhage group and control group （n=40）; 20 μL arterial blood from the tail arteries or normal saline were injected into the caudate nucleus of intracerebral hemorrhage group and control group by stereotactic technique,respectively.One,three,five and seven d after injection,the neurological impairment was scored; the behavioral changes of the mice in the Morris water maze （navigation test and space exploration experiment） were observed; brain edema was measured by wet and dry weight method and electrophysiological differences of hippocampal neurons were recorded by whole-cell patch-clamp technique and computer software.Results As compared with those in the control group,significantly increased neurological deficit scores one,three,five and seven d after injection,statistically decreased residence time in the platform on the fifth d of training,obviously increased water content around the brain edema one,three,five and seven d after injection,and significantly decreased resting membrane potential and input resistance in the hippocampal CA1 pyramidal cells five d after injection of mice in the intracerebral hemorrhage group were noted （P〈0.05）.Conclusion The hippocampus-dependent spatial leaming ability of intracerebral hemorrhage mice is decreased,and the permeability of potassium channels is enhanced.

关 键 词：脑出血 海马 行为学 脑水肿 电生理特性 

分 类 号：R743.34[医药卫生—神经病学与精神病学]