兔关节盘、髁突及软骨下骨的年龄相关性改变组织学研究  

Age-related Changes of Condyle, Articular Disc, and Subchondral Bone:a Histomorphometric Study in Rabbit Temporomandibular Joint

作　　者：黄林剑[1] 蔡协艺[1] 李辉[1] 盛夏涵 杨驰[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔外科.上海市口腔医学重点实验室,上海200011

出　　处：《口腔颌面外科杂志》2015年第1期19-23,共5页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(81200766);上海市科学技术委员会资助项目(08DZ2271100;114119a3800;13140902702;13XD1402300)

摘　　要：目的：探究不同年龄段兔颞下颌关节（TMJ）关节盘、髁突和软骨下骨的生理特征的变化。方法 ：选取1、4、12和32周龄健康雌性新西兰大白兔各3只,取材TMJ,经脱钙处理后进行5μm连续切片,苏木精-伊红染色。分别对关节盘大小、髁突前后径,以及关节盘所对应软骨下骨的形态结构和骨密度进行测量分析。采用SPSS19.0软件包对数据进行统计学分析。结果：关节盘大小比较,4周组明显大于1周组（P=0.039）,12周组明显大于4周组（P=0.007）;髁突前后径在4周和12周组间（P=0.003）显著增加;软骨下骨的形态结构和骨密度在4~32周内（P〈0.05）显著改变。结论：兔颞下颌关节关节盘和髁突在青春期前生长发育明显;髁突软骨下骨的形态结构和骨密度在4周时开始出现明显改变。虽然青少年期和成年期兔髁突大小没有统计学差异,但是两者软骨下骨的骨质密度差异明显。Objective： To study the age-related changes of articular disc, condyle and subchondral bone in rabbit temporo- mandibular joint （TMJ）. Methods： Three New Zealand rabbit in each group were obtained at the ages of 1-,4-,12- and 32- week, respectively. Animals were sacrificed and TMJ specimen was subjected to histolgical assessment to evaluate about the length of articular disc and condyle and the architecture of subchondral bone. One-way ANOVA using SPSS 19.0 soft- ware package was performed. Results： Articular disc significant elongated from 1- week to 4- week rabbits （P=0.039） and 4- week to 12- week rabbits （P=0.007）. The increase of subchondral bone density （P〈0.05） occurred from 1- week to 4- week and 4- week to 32- week rabbits respectively. The significant enlargement of condyle length occurred from 4- week to 12- week rabbits （P=-0.003）. Conclusion： The growth and development potential of the articular disc and the mandibular condyle were promintly before the adolesence. The morphology structure and bone mineral density of subchondral bone changed from 4 to 32 weeks after birth, resulting in the condyle enlargement at first and the increase of bone density after- wards. In additon, there was no significant changes in condylar cartilage between adolescence and adulthood. However, the architecture of the adulthood subchondral bone was much stronger than the adolescence condyle. These provided histologi- cal basis for adulthood mandibular condyle had a stronger adaptive capacity to alteration of pressure microenvironment in temporomandibular joint.

关 键 词：兔 颞下颌关节 关节盘 髁突 软骨下骨 生长发育 骨密度 

分 类 号：R782.6[医药卫生—口腔医学]