快速原型技术制备Nano-HA/PCL支架与犬骨髓基质干细胞体外复合研究  

Rapid Prototyping of Nano-HA/PCL Composite Scaffolds and Osteogenic Potential of Bone Marrow Stroma Cells of Canine in vitro

作　　者：郭凌云[1] 张劲娥[1] 袁建兵[2] 黄远亮[1] 

机构地区：[1]上海市东方医院口腔科,上海200120 [2]上海交通大学机械与动力工程学院,上海200030

出　　处：《口腔颌面外科杂志》2015年第1期34-38,共5页Journal of Oral and Maxillofacial Surgery

基　　金：上海市浦东新区卫生局优青培养计划(PWRq2011-03)

摘　　要：目的:探讨快速原型制作纳米-羟基磷灰石/聚己内酯(nano-HA/PCL)根形三维支架的细胞生物相容性,为进一步体内实验提供研究基础。方法:通过CT扫描获得犬头颅骨影像信息,以CAD软件实现犬下颌牙根形态的三维重建影像,应用快速原型(RP)技术制作nano-HA/PCL根形支架,经犬骨髓基质细胞接种后体外复合培养,检测支架材料的细胞相容性。结果:通过CT影像和快速原型技术,可实现解剖根形态结构nano-HA/PCL三维仿真支架。细胞支架复合培养后,扫描电镜显示细胞生长附着于支架表面;细胞与支架复合后增殖情况良好;7 d和14 d碱性磷酸酶活性高于对照组。结论:体外复合培养结果表明,RP技术制备的nano-HA/PCL支架和骨髓基质细胞生物相容性较好,可进一步应用于牙槽骨缺损骨组织工程修复动物实验。Objective： To fabricate nano-hydroxyapatite/Poly-e-caprolactone（nano-HA/PCL） scaffold with three-dimensional simulation of the root shape by using a rapid prototyping （RP） process. To evaluate biocompatibility of the scaffold used for bone tissue engineering. Methods： Parietal bone image information of canine was obtained by CT scan, three-dimensional reconstruction images were performed by CAD, roots shape nano-HA/PCL scaffolds was obtained by RP technology. Canine 3rd passage bone marrow stromal cells （BMSCs） were seeded in nano-HMPCL scaffolds and grown in osteogenic medium. Cells were harvested after 1, 3, and 7 days. BMSCs proliferation and ALP activity were measured. Results： Scanning electron micrographs showed the BMSCs adhered to the inner surface of the scaffolds. Cell proliferation steadily increased with time, and alkaline phosphatase activity in the scaffolds at 7- day and 14- day were enhanced. Conclusion： The os- teogenic potential of BMSCs seeded at the rapid prototyped nano-HA/PCL scaffolds demonstrate a good biocompatibility, and may be effective for animal experiment in alveolar bone repair in the near future.

关 键 词：快速原形技术 羟基磷灰石/聚己内酯支架 骨髓基质细胞 生物相容性 犬 

分 类 号：R783.1[医药卫生—口腔医学]