全脑缺血大鼠脑组织MiR210和stat3的表达与HIF-1α的相关性  

Expressions of MiR210 and stat3 following global ischemia in rats and the potential relationship among MiR210,stat3 and HIF-1α

作　　者：范少华[1] 张轶超[2] 袁晓东[2] 毕景雯 张一辰 李云[2] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东大学附属济南市中心医院重症医学科,山东济南250013

出　　处：《山东大学学报（医学版）》2015年第3期1-5,11,共6页Journal of Shandong University:Health Sciences

基　　金：济南市科技发展计划(201401083)

摘　　要：目的研究全脑缺血再灌注损伤大鼠海马信号转导和转录激活因子3(stat3)及大脑皮层MiR210的表达变化,探讨MiR210及stat3与缺氧诱导因子-1α(HIF-1α)之间的相关性及调节机制。方法将66只SD大鼠随机分为假手术组、全脑缺血组(GI组)及stattic+全脑缺血组(stattic+GI组)。采用改良四血管阻断法制备大鼠全脑缺血模型,免疫印迹法检测海马stat3、p-stat3及HIF-1α蛋白的表达,实时定量PCR法检测大脑皮层MiR210及HIF-1αmRNA的变化。结果与假手术组相比,GI组海马stat3、p-stat3及HIF-1α蛋白表达均明显增加(P<0.05),大脑皮层M iR210及HIF-1αmRNA表达亦明显增加(P<0.05);与GI组相比,stattic+GI组海马p-stat3及HIF-1α蛋白表达均明显减少(P<0.05),而stat3蛋白变化不明显(P>0.05);大脑皮层HIF-1αmRNA水平明显减少(P<0.05)。结论全脑缺血再灌注损伤后,MiR210及stat3表达发生明显变化,且两者与HIF-1α之间可能存在调控关系。Objective To study the expression of signal transducer and activator of transcription 3（ stat3） in hippocampus and the expression of MiR210 in cerebral cortex following global ischemia in rats,and to explore the potential relationship among MiR210,stat3 and hypoxia inducible factor-1α（ HIF-1α）. Methods Sixty-six SD rats were randomly divided into three groups： sham-operated group,global ischemia group（ GI group）,and stattic ＋ global ischemia group（ stattic ＋ GI group）. The global ischemia model was induced by modified four-vessel occlusion method. Stat3,p-stat3 and HIF-1α protein expressions in hippocampus were detected by Western blotting. MiR210 and HIF-1α mRNA expressions in cerebral cortex were detected by Real-time PCR. Results Compared with sham-operated group,stat3,p-stat3 and HIF-1α protein expressions in hippocampus and MiR210 and HIF-1α mRNA expressions in cerebral cortex of GI group significantly increased（ all P〈0. 05）. Compared with GI group,p-stat3 and HIF-1α protein expressions in hippocampus of stattic ＋ GI group significantly decreased,stat3 protein expression showed no statistical difference（ P〉0. 05）,and HIF-1α mRNA expression in cerebral cortex signifcantly decreased（ P〈0. 05）. Conclusion Expressions of MiR210 and stat3 have a marketable change after global ischemia reperfusion,and regulatory relationships may exist among stat3,MiR210 and HIF-1α.

关 键 词：全脑缺血 大鼠 MiR210 信号转导和转录激活因子3 缺氧诱导因子-1Α 

分 类 号：R743.31[医药卫生—神经病学与精神病学]