黑素皮质素受体3基因(MC3R)多态性及其单倍型组合与京海黄鸡屠体性状的关联分析  

Polymorphism of Melanocortin 3 Receptor Gene(MC3R) and Association Analysis Between the Diplotypes and the Carcass Traits in Jinghai Yellow Chicken

作　　者：薛倩[1] 王金玉[1] 张跟喜[1] 张涛[1] 王文浩[1] 段炼[1] 韩昆鹏[1] 王永娟 

机构地区：[1]扬州大学动物科学与技术学院/江苏省动物遗传繁育与分子设计重点实验室,扬州225009 [2]江苏京海集团,南通22610

出　　处：《农业生物技术学报》2015年第3期344-351,共8页Journal of Agricultural Biotechnology

基　　金：国家肉鸡产业技术体系(nycytx-42-G1-05);江苏高校优势学科建设工程;江苏省动物遗传繁育与分子设计重点实验室

摘　　要：本研究旨在探讨黑素皮质素受体3基因(melanocortin 3 receptor,MC3R)对鸡(Gallus gallus)屠体性状的影响。采用聚合酶链式反应-单链构象多态性(polymerase chain reaction-single strand conformation polymorphism,PCR-SSCP)检测及测序的方法,检测MC3R在京海黄鸡群体中的单核苷酸多态性(single nucleotide polymorphisms,SNPs),并对这些多态位点进行连锁不平衡和单倍型分析。结果表明,在MC3R编码区序列(coding sequence,CDS)共检测到6个SNPs位点,各位点间均不存在强连锁不平衡;6个SNPs位点在379只京海黄鸡的群体中形成了5种单倍型和11种单倍型组合。最小二乘法分析结果表明,不同单倍型组合间京海黄鸡屠体性状(包括活体重、屠体重、胸肌重、腿肌重、全净膛重、半净膛重和腹脂重)存在显著或极显著差异(P<0.05或P<0.01),其中单倍型组合H3H4和H3H3的各种屠体性状均极显著高于单倍型组合H4H5(P<0.01),也显著高于其他部分单倍型组合(P<0.05),为屠体性状优势组合;单倍型组合H4H5各屠体性状均为最低,均显著或极显著低于其他大部分单倍型组合(P<0.05或P<0.01),为屠体性状劣势组合。因此,MC3R可能是影响鸡屠体性状的主效基因或与主效基因连锁,本研究为京海黄鸡屠体性状分子标记辅助选择提供了参考依据。The object of this study was to analyze the effects of melanocortin 3 receptor （MC3R） gene on chicken＇s （Callus gallus） carcass traits. The single nucleotide polymorphisms （SNPs） of MC3R in Jinghai Yellow chicken were detected by polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） combined with sequencing technology. Then the linkage disequilibrium （LD） and haplotype analysis were conducted. As a result, 6 SNPs were identified in the coding region of MC3R, which were C160A, G192T, C273T, G310A, G549A and G762A, respectively, and there＇s no strong LD between the SNPs. It was found that among the 6 SNPs, the mutation C160A resulted in an amino acid change from Leu to Met, and the mutation G310A resulted in an amino acid change from Gly to Ser, and the other 4 SNPs （including G192T, C273T, G549A and G762A） all resulted in none amino acid changes which were synonymous mutations. Five haplotypes and 11 diplotypes were formed in the group of 379 Jinghai Yellow chicken. The least square analysis showed that there were significant or highly significant differences （P〈0.05 or P〈0.01） in the carcass traits of Jinghai Yellow chicken （including live weight, carcass weight, breast muscle weight, leg weight, eviscerated weight, half eviscerated weight and abdominal fat weight） between the 2 different diplotypes. For the live weight of Jinghai Yellow chicken, the difference between diplotype H4H5 and H4H4 was not significant （P〉 0.05）, but both of them were significantly or highly significantly （P〈0.05 or P〈0.01） lower than other diplotypes. In the aspect of carcass weight, H3H3 and H3H4 diplotypes had the highest value which were significantly or highly significantly （1~〈0.05 or P〈0.01） higher than other diplotypes, and H4H5 diplotype had the minimum carcass weight which were significantly or highly significantly （P〈0.05 or P〈0.01） lower than other diplotypes. The breast muscle weight of H3H3 and H3H4 diplotypes was significantly （

关 键 词：京海黄鸡 黑素皮质素受体3基因(MC3R) 屠体性状 单核苷酸多态性(SNPs) 单倍型分析 

分 类 号：S831.2[农业科学—畜牧学]