细胞周期调控因子P16^(INK4)、P57^(kip2)和Ki67在肝癌中的表达及意义  

Expression and significance of P16^(INK4),P57^(kip2) and Ki67 in hepatocellular carcinoma

作　　者：高涵[1] 周涛 张春晶[1] 李淑艳[1] 王丽萍[1] 刘哲丞[1] 

机构地区：[1]齐齐哈尔医学院生物化学与分子生物学教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医院第一附属医院普通外科,黑龙江齐齐哈尔161006

出　　处：《重庆医学》2015年第9期1165-1167,共3页Chongqing medicine

基　　金：黑龙江省齐齐哈尔市科学技术计划基金资助项目(SFGG-201338)

摘　　要：目的探讨细胞周期调控因子P16INK4、P57kip2和Ki67在肝癌中的表达水平及其意义。方法收集正常肝组织标本21例,肝细胞肝癌标本48例及对应的癌旁组织标本48例为研究对象,通过免疫组织化学和Western blot分析P16INK4、P57kip2和Ki67的表达水平。结果 P16INK4及P57kip2蛋白肝癌组织中的表达与癌旁组织及正常肝脏组织比较显著降低,阳性率分别为49.3%和34.2%,Ki67蛋白肝癌组织中的表达水平显著升高,其阳性率为91.7%,与癌旁组织及正常肝脏组织比较差异均有统计学意义(P<0.05),结果与Western blot结果一致;高、低分化组P57kip2、Ki67及P16INK4的表达水平比较差异均有统计学意义(P<0.05)。结论 P16INK4、P57kip2表达降低及Ki67的高表达在肝细胞癌的发生、发展过程中可能有重要的作用。Objective Analysis the expression and significance of P16INK4 ,P57kip2 and Ki67 in hepatocellular carcinoma .Meth-ods 21 normal liver tissue ,48 hepatocellular carcinoma tissues and 48 corresponding adjacent tissue were collected for this study . Immunohistochemical analysis the expression levels of P16INK4 ,P57kip2 and Ki67 .Results P16INK4 and P57kip2 in hepatocellular carci-noma tissue were significantly lower ,the positive rates were 49 .3% and 34 .2% respectively ,Ki67 was significantly higher than the normal liver tissue and corresponding adjacent tissue ,the difference was statistically significant（P〈0 .05） ,the results were consist-ent with the Western blot ;different differentiated group p57kip2 ,Ki67 expression and P16INK4 were significant differences（P〈0 .05） . Conclusion Low expression of P16INK4 and P57kip2 ,the higher expression of Ki67 play an important role in the development of hepa-tocellular carcinoma .

关 键 词：P16INK4 P57KIP2 KI67 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]