非小细胞肺癌EGFR基因突变与ERCC1、TYMS mRNA表达相关性的研究  

Correlation of EGFR mutation with ERCC1 and TYMS mRNA expression in non-small cell lung cancer

作　　者：张全[1] 谭群友[1] 王如文[1] 陶绍霖[1] 康珀铭[1] 邓波[1] 周景海[1] 李坤[1] 钱凯[1] 蒋彬[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所胸外科,重庆400042

出　　处：《重庆医学》2015年第9期1177-1179,1183,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81172239)

摘　　要：目的：探讨非小细胞肺癌表皮生长因子受体（EGFR）基因突变与核苷酸切除修复交叉互补基因1（ERCC1）、胸苷酸合成酶（TYMS） mRNA表达的相关性。方法收集该科2013年2～12月符合入组条件的NSCLC患者97例，肿瘤组织标本由术中切取或穿刺获得。使用xTAG-液相芯片法检测EGFR（E18、E19、E20、E21）基因突变，使用分支DNA-液相芯片法检测ER-CC1、TYMS mRNA表达水平，并分析EGFR基因突变与 ERCC1、TYMS mRNA表达水平的相关性。结果97例检测样本中， EGFR基因突变29例，突变率为30％（29／97），女性、无吸烟史、腺癌患者EGFR基因突变检出率较高（P＜0．05）。EGFR突变与ERCC1 mRNA表达有相关性（χ2＝4．088，P＜ 0．05），与 TYMS mRNA 表达无相关性（χ2＝ 0．265，P＞ 0．05）。结论 NSCLC EGFR基因突变与ERCC1 mRNA表达存在相关性，与TYMS mRNA表达无相关性。Objective To investigate whether EGFR gene mutations are correlated with the gene expression of ERCC1 and TYMS in non-small-cell lung cancer .Methods Collected February to December 2013 of non-small cell lung cancer（NSCLC） pa-tients eligible for enrolled 97 patients ,tumor tissue specimens obtained by intraoperative cut or puncture ,Gene expression of ERCC1 and TYMS were determined by branched-DNA liquid chip ,while somatic mutations in EGFR（E18 ,E19 ,E20 ,E21） gene were detec-ted by xTAG-liquid chip;And analysis of EGFR gene mutation associated with ERCC1 ,TYMS mRNA expression .Results Totally 29 cases of EGFR mutation were detected in all 97 specimens ,with a mutation rate of 30% （29/97） ,and a relatively high detection rate was observed in female ,adenocarcinoma and non-smoking patients（P〈0 .05） .EGFR mutation was relevant to the expression of ERCC1（χ2 =4 .088 ,P〈0 .05） ,EGFR mutation was irrelevant to the expression of TYMS（χ2 =0 .265 ,P〈0 .05） .Conclusion In NSCLC tissues ,EGFR mutation is relevant to the expression of ERCC1 but irrelevant to the expression of TYMS .

关 键 词：非小细胞肺癌 核苷酸切除修复交叉互补基因1 胸苷酸合成酶 表皮生长因子受体 

分 类 号：R734.2[医药卫生—肿瘤]