黏膜相关淋巴组织结外边缘区淋巴瘤病理特征、IgM表达及与MYD88 L265P突变的相关性研究  

Pathological features,IgM expression and MYD88 L265P mutation in MALT lymphoma

作　　者：方建晨 周晓燕[2] 王朝夫[2] 李征 李海莉 

机构地区：[1]宁波市临床病理诊断中心病理科,315031 [2]复旦大学附属上海市肿瘤医院病理科

出　　处：《浙江医学》2015年第3期205-207,221,共4页Zhejiang Medical Journal

基　　金：宁波市自然科学基金(2012A610200)

摘　　要：目的探讨不同部位黏膜相关淋巴组织结外边缘区淋巴瘤(MALT淋巴瘤)的病理特征、并分析IgM表达与MYD88 L265P突变的相关性。方法对发生在不同部位的65例MALT淋巴瘤患者的标本进行病理组织学及免疫组化分析,选取其中可行分子生物学研究的26例标本进行IgM表达检测,以测序法检测了MYD88 L265P的突变状况,并研究了IgM表达与MYD88 L265P突变间的相关性。结果65例患者的病变部位包括胃18例、涎腺10例、肺9例、肠道8例、眼7例、甲状腺5例、其他部位8例。不同器官的病变具有相似的组织学和大部分免疫组化表达,但Bcl-2的表达在肺(6/9)和甲状腺(2/5)与总体(56/65)相比均有统计学差异(均P>0.05)。26例标本中有16例IgM呈阳性,而发生MYD88 L265P的突变的病例只有1例,两者无相关性(P=0 615)。结论发生在不同的部位的MALT淋巴瘤具有相似的病理学特征,MYD88 L265P的突变率在MALT淋巴瘤较低,且与IgM表达无明显相关性。Objective To investigate the pathological features, IgM expression and MYD88 L265P mutation of extran-odal marginal zone B cel lymphoma （MALT lymphoma）. Methods The clinical and pathological data of 65 cases of MALT lymphoma were col ected and analyzed. MYD88 gene mutation was determined by PCR and DNA sequencing, IgM expression was detected by immunohistochemistry in 26 cases of MALT lymphoma. Results MALT lymphoma was initial y diagnosed in stomach （n=18）, salivary glands （n=10）, lung （n=9）, intestine （n=8）, conjunctiva （n=7）, thyroid （n=5） and other sites （n=8）. The histological and immunophenotypic features of the lymphoma involving different organs were similar. Among 26 cases MYD88 L265P mutation was detected in 1 case and IgM was expressed in 16 cases. Conclusion MALT lymphomas involving different organs have similar pathological features. The frequency of MYD88 L265P mutation is low, and the correlation between MYD88 gene mutation and IgM expression is not found.

关 键 词：MYD88 L265P突变 黏膜相关淋巴组织结外边缘区淋巴瘤 

分 类 号：R733.1[医药卫生—肿瘤]