2型糖尿病患者尿液的代谢组学研究  

Urinary metabonomics in type 2 diabetic patients

作　　者：苏君梅[1] 葛卫红[1] 许广艳[1] 张宇[2] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江省中医院内科

出　　处：《浙江医学》2015年第4期278-282,共5页Zhejiang Medical Journal

基　　金：浙江省钱江人才计划(2010D003)

摘　　要：目的从代谢组学角度分析并寻找2型糖尿病(T2DM)患者可能的代谢标记物。方法选取30例初诊、或有糖尿病史经药物治疗控制不理想且无并发症的T2DM患者,另选取30例性别、年龄匹配的健康者为正常对照。收集清晨空腹中段尿,以气相色谱-质谱联用(GC-MS)技术对尿液样本进行代谢图谱分析,正交偏最小二乘法判别研究尿液内源性化合物在两组间的差异。结果 T2DM组和正常对照组尿液代谢谱明显分离。与正常对照组比较,T2DM组尿液2,3,4-三羟基丁酸、肌醇、D-葡萄糖、D-葡萄糖酸及尿素含量升高(P<0.05或0.01),马尿酸含量减少(P<0.01)。结论代谢组学检查提示T2DM患者尿液中代谢标志物为2,3,4-三羟基丁酸、肌醇、马尿酸、D-葡萄糖、D-葡萄糖酸及尿素,观察这些标志物含量的变化有助于T2DM的临床诊断及发病机制研究。Objective To investigate the urinary metabonomics in patients with type 2 diabetes mellitus. Methods Thirty patients with type 2 diabetes mellitus were enrolled as study group, and 30 health subjects served as controls. The morning midstream urine samples were collected. Gas chromatography-mass spectrometry（GC-MS） was used to analyze metabonomics of the urine samples. Orthogonal partial least-squares discriminant analysis（OPLS-DA） was applied to identify potential biomarkers. Results The difference of metabonomics spectra was observed between diabetic patients and normal controls. Compared with the normal controls, the contents of 3-hydroxybutyrate, inositol, D-glucose, D-gluconic acid, urea were increased （P〈0.05 or P〈0.01 ） and hippuric acid decreased significantly （P〈0.01） in type 2 diabetic patients. Conclusion The 3-hydroxybutyrate, inositol, hippuric acid, D-glucose, D-gluconic acid and urea in the urine might be the candidate biornarkers for type 2 diabetes mellitus.

关 键 词：代谢组学 2型糖尿病 气相色谱-质谱联用 正交偏最小二乘法判别 

分 类 号：R587.1[医药卫生—内分泌]