藤茶主要成分对胰岛素抵抗HepG2细胞内氧化应激水平的影响  

Effects of main compounds from Ampelosis grossedentata on oxidative stress in insulin-resistant HepG2 cells

作　　者：潘慧敏[1,2] 姜保平[1,2] 乐亮[1] 肖伟[1,2] 许利嘉[1,2] 肖培根[1,2] 

机构地区：[1]中国医学科学院 北京协和医学院药用植物研究所,北京100193 [2]国家教育部中草药物质基础与资源利用重点实验室,北京100193

出　　处：《中国现代中药》2015年第3期267-271,共5页Modern Chinese Medicine

基　　金：国家自然科学基金面上项目(81274188):四种别样茶改善胰岛素抵抗的物质基础和作用机制研究

摘　　要：目的:探讨藤茶主要成分,二氢杨梅素、没食子酸、二氢槲皮素、杨梅素及杨梅苷对胰岛素抵抗HepG2细胞糖消耗及细胞内活性氧(ROS)、丙二醛(MDA)、乳酸(LD)、三磷酸腺苷(ATP)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响。方法:将HepG2细胞分为正常对照组、胰岛素抵抗模型组和样品干预组。用高糖(55 mmol·L-1)诱导HepG2细胞,建立胰岛素抵抗模型,用MTT法检测样品对HepG2细胞增殖的影响;用葡萄糖氧化酶法检测上清中葡萄糖的含量;用分光光度法检测细胞内MDA、LD含量及SOD、CAT活性;用荧光酶标仪检测细胞内ROS和ATP水平。结果:五个化合物在有效浓度范围内对HepG2细胞增殖无显著影响;样品干预组葡萄糖消耗量较模型组显著升高;与模型组相比,五个化合物某个或某几个浓度组显著降低细胞内MDA含量,提高SOD、CAT活性及ATP水平;没食子酸、二氢槲皮素和杨梅苷显著降低细胞内ROS水平;除二氢杨梅素外,其余四个化合物均能降低细胞内LD含量。结论:胰岛素抵抗HepG2细胞与对照组细胞相比有较高的氧化应激水平,抗氧化能力减弱。藤茶主要成分能通过降低细胞的氧化应激水平,增加细胞的抗氧化能力,从而预防胰岛素抵抗。Objective： To investigate the influence of the main compounds： dihydromyricetin, gallic acid, dihydroquercetin, myricetin and myricitrin from Ampelopsis grossedentata on glucose consumption, ROS, MAD, SOD, CAT, LD and ATP in insulin-resistant HepG2 cells. Methods： HepG2 ceils were divided into control group, model group and sample groups. Insulin-resistant cells model was induced by high concentration of glucose （55 mmol· L ^- 1 ）. The proliferation of cells were detected by 3-（4, 5-dimethyhhiazol-2-yl）-2, 5-diphenyherazolium bromide （MTF） assay. The contents of glucose in culture supematant were measured by the glucose oxidase peroxides （GOD） method. The contents of MDA, SOD, CAT and LD were observed by spectrophotometric method. The contents of ROS and ATP were analyzed on a fluorescence microplate. Results： The five compounds had little impact on the cells proliferation within the range of effective concentration. The five compounds increased the glucose consumption significantly in insulin-resistant cells. After treated with the five compounds, the contents of SOD, CAT and ATP increased significantly, while that of MDA decreased. Dihydroquercetin, gallic acid and myricitrin were able to reduce the contents of ROS and LD significantly. Meanwhile, the content of LD also could be reduced significantly by myricetin. Conclusion： Dihydromyricetin, gallic acid, dihydroquercetin, myricetin and myrieitrin from A. grossedentata can alleviate oxidative stress in the insulin- resistant HepG2 cells.

关 键 词：藤茶 HEPG2细胞 胰岛素抵抗 氧化应激 

分 类 号：R273.57[医药卫生—中西医结合]