Toll样受体2在口腔鳞癌中的表达及其作用机制  

Expression of Toll-like receptor 2 in oral squamous cell carcinoma and its mechanism

作　　者：王海欣 吴景华[2] 陈继科 郭谊 时建华 

机构地区：[1]唐山市中医医院口腔科,唐山063000 [2]河北联合大学附属医院检验科 [3]唐山市协和医院口腔科

出　　处：《山西医科大学学报》2015年第3期227-230,282,共5页Journal of Shanxi Medical University

基　　金：唐山市科技局课题资助项目(14130262a)

摘　　要：目的探讨TLR2在临床口腔鳞癌患者中的表达情况,以及TLR2在口腔鳞癌发生和发展过程中的作用,进而为临床口腔鳞癌的干预和治疗寻找新的治疗途径。方法无菌提取24例口腔鳞癌患者、24例良性肿瘤患者及24例对照组外周血单核细胞,利用实时定量PCR及Western blot方法检测TLR2的表达情况,同时通过免疫组化技术来检测癌组织中的TLR2表达水平的差异;通过TLR2激动剂(弗氏完全佐剂)及弗氏不完全佐剂(阴性对照组)作用口腔鳞癌成瘤小鼠,观察其瘤体的生长及荷瘤小鼠的存活情况。结果口腔鳞癌患者外周血单核细胞中TLR2的表达较之对照组明显增加(P<0.05),同时TLR2表达水平在口腔鳞癌组织中也明显增强。注射弗氏佐剂组的小鼠瘤体的体质量为(1.42±0.43)g,较之空白对照组[(2.47±0.69)g]及阴性对照组(弗氏不完全佐剂组)[(2.94±0.87)g]明显降低,同时小鼠的生存期也明显延长(P<0.05)。结论口腔鳞癌患者外周血单核细胞及癌组织中TLR2的表达明显升高,同时TLR2的高表达具有明显的肿瘤抑制作用。Objective To explore the expression of Toll-like receptor 2（ TLR2） in oral squamous cell carcinoma（ OSCC） and its role in the development of tumor. Methods The expression of TLR2 was detected in peripheral monocyte of 24 cases of OSCC,24 cases of benign tumor and 24 normal controls by RT-PCR and Western blot. And the TLR2 expression in tumor tissues was detected by immunohistochemical method. Freund＇s complete adjuvant（ CFA） and incomplete adjuvant（ IFA） were injected into mice to observe the survival time of tumor-bearing mice and the tumor growth. Results The expression of TLR2 mRNA in peripheral monocyte was higher in OSCC than those in normal controls or in benign tumor（ P〈0. 05）. And the TLR2 protein levels were also higher in tumor tissues of OSCC than in normal controls or in benign tumor（ P〈0. 05）. The tumor weight was（ 1. 42 ± 0. 43） g in mice injected with CFA,（ 2. 94± 0. 87） g in mice injected with incomplete adjuvant（ IFA） and（ 2. 47 ± 0. 69） g in blank control mice. The survival time of the tumorbearing mice in CFA group was significantly prolonged compared with IFA group and blank group（ P〈0. 05）. Conclusion TLR2 expression increases in peripheral monocyte and tumor tissues of OSCC patients,and TLR2 high expression significantly inhibit the proliferation of human tongue squamous cancer cells.

关 键 词：口腔鳞癌 TLR2 免疫组化 

分 类 号：R739.8[医药卫生—肿瘤]