CK、P63与原位杂交EBER在诊断复发性鼻咽癌中的应用  

Application of Cytokeratin,P63 and in-situ-hybridization EBER in diagnosis of recurrent nasopharyngeal carcinoma

作　　者：陈应智[1] 储兵[1] 曾玉梅[1] 曹晓珊[1] 

机构地区：[1]广东省中山市人民医院病理科,528400

出　　处：《齐齐哈尔医学院学报》2015年第6期787-789,共3页Journal of Qiqihar Medical University

摘　　要：目的探讨免疫组化CK、P63与原位杂交EBER在诊断复发性鼻咽癌的应用。方法收集54例临床怀疑鼻咽癌复发病例,分别进行CK、P63及原位杂交EBER检测。结果 54例中有40例为复发癌,14例为炎症或放疗后改变。40例复发癌CK、P63均阳性(100%),38例EBER阳性(95%),而14例非复发癌CK、P63及EBER均为阴性(100%),两组阳性率对比有统计学差异(P<0.05)。40例复发癌中2例为角化型鳞癌(5%),非角化型分化性癌4例(10%),非角化型未分化性癌34例(85%),且复发组织学类型与原发癌类型一致。结论结合形态学,免疫组化CK、P63及原位杂交EBER,可以对是否为鼻咽癌复发作出明确诊断。Objective To investigate application of Cytokeratin （CK）, p63 and Epstein-Barr virus- encoded small RNAs（EBER） by immunohistochemistry staining and in situ hybridization in diagnosis of recurrent nasopharyngeal carcina（rNPC）. Methods By collecting the clinical suspicion of 54 cases ofrNPC, to CK,P63 and in situ hybridization EBER detection respectively. Results 40 cases were recurrence of cancer, 14 cases of inflammation or change after radiotherapy. 40 cases of rNPC CK and P63 were positive （ 100% ）, 38 cases of EBER positive （95%） ,14 cases of non recurrent cancer CK, P63 and EBER were negative （ 100% ）. The two groups had statistical difference between the positive rate of contrast （P 〈 0. 05 ）. There were 2 cases of keratinizing squamous cell carcinoma（5% ） in 40 cases of recurrent carcinoma . Non cornification differentiated carcinoma in 4 cases （ 10% ）, type non cornification undifferentiated carcinoma with 34 cases （85%）. and the Recurrent carcinoma histological types and primary cancer types are the same. Conclusions Combined with the morphological, immunohistochemical CK, P63 and EBER in situ hybridization, can clear diagnosis to make whether for recurrent nasopharyngeal carcinoma.

关 键 词：细胞角蛋白 P63 原位杂交 复发性鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]