人参皂苷Rg1对帕金森病小鼠黑质多巴胺能神经元凋亡及EphB1、TH、P-c-Jun蛋白表达的影响  

Effects of ginsenoside Rg1 on apoptosis of dopamine neurons,expressions of Eph B1,TH and P-c-Jun in substantia nigra of mice with Parkinson's disease

作　　者：王淑秀[1] 常海敏[2] 朱丰霞 李培艳[1] 段瑛[1] 

机构地区：[1]新乡医学院病理学教研室,新乡453003 [2]新乡医学院法医临床学教研室,新乡453003

出　　处：《郑州大学学报（医学版）》2015年第2期176-180,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高校科技创新人才支持计划资助项目2010HASTIT008

摘　　要：目的:观察人参皂苷Rg1对MPTP所致帕金森病(PD)模型小鼠黑质多巴胺能神经元凋亡及酪氨酸羟化酶(TH)、Eph B1和P-c-Jun蛋白表达的影响。方法:将27只C57BL/6小鼠等分为对照组、模型组和人参皂苷Rg1组。腹腔注射人参皂苷Rg1预防给药3 d,腹腔注射MPTP构建PD模型。观察不同时间点小鼠游泳情况。MPTP给药2周后采用TUNEL染色检测小鼠黑质多巴胺能神经元凋亡情况,采用免疫组化检测黑质Eph B1蛋白的表达,采用Western blot检测黑质TH、P-c-Jun蛋白的表达。结果:模型组小鼠不同时间点游泳实验分值低于对照组和人参皂苷Rg1组(P均<0.05)。模型组黑质多巴胺能神经元凋亡数、Eph B1阳性细胞数和P-c-Jun蛋白相对表达量均高于对照组和人参皂苷Rg1组,而模型组黑质TH蛋白相对表达量低于对照组和人参皂苷Rg1组(P均<0.05)。结论:人参皂苷Rg1可能通过减少黑质多巴胺能神经元凋亡以及降低Eph B1、P-c-Jun的表达而改善PD的症状。Aim： To explore the influence of ginsenoside Rgl on apoptosis of dopamine neurons, expressions of TH, EphB1 and P-c-Jun proteins in substantia nigra of mice with Parkinson＇s disease（PD） induced by MPTP. Methods： Twenty-seven C57BL/6 mice were randomly divided into control group, model group and ginsenoside Rgl group. The mice of ginsenoside Rgl group were pretreated with ginsenoside Rgl. MPTP was injected intraperitoneally to mice to make PD model. The swimming test of mice was determined at different time points. Two weeks later, apoptosis of dopamine neurons in substantia nigra of the mice was determined by TUNEL staining. The expression of EphB1 protein in substantia nigra of the mice was detected by immunohistochemistry. The expressions of TH and P-e-Jun proteins in substantia nigra of the mice were detected by Western blot. Results： The swim-score values of model group were significantly lower than those of control group and ginsenoside Rgl group at different time points（P 〈 0.05）. The number of apoptosis dopamine neurons, the positive expression of EphB1 protein, and the relative expression of P-c-Jun protein of the model group were higher than those of control group and ginsenoside Rgl group（ P 〈 0.05 ）. The relative expression of TH of model group was significantly lower than those of the control group and ginsenoside Rgl group（ P 〈 0.05 ）. Conclusion： Ginsenoside Rgl may improve the symptoms of PD through decreasing apoptosis of dopamine neurons, and reducing the expressions of EphB1 and P-c- Jun.

关 键 词：帕金森病 人参皂苷RG1 凋亡 Eph B1 P-c-Jun 小鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]