雄激素调节耐比卡鲁胺前列腺癌细胞肺耐药蛋白的表达  

Expression of lung resistance protein in bicalutamide-resistant human prostate cancer cells regulated by androgen

作　　者：朱文胜[1] 谭毅[1] 杨松[1] 邹飞[1] 陈勇[1] 张洋[1] 彭拓[1] 

机构地区：[1]重庆三峡医药高等专科学校附属医院泌尿外科,重庆404100

出　　处：《海南医学》2015年第5期635-637,共3页Hainan Medical Journal

摘　　要：目的探讨雄激素对耐比卡鲁胺前列腺癌细胞肺耐药蛋白(LRP)表达的调节作用,为前列腺癌病理及临床的深入研究提供理论依据。方法选用PC-3和LNCa P细胞株,建立耐比卡鲁胺前列腺癌细胞株,并以二氢睾酮(DHT)对耐比卡鲁胺前列腺癌细胞株诱导,采用免疫细胞化学法和Western Blot检测LRP的表达状况。结果耐比卡鲁胺前列腺癌细胞株的LRP表达相对前列腺癌细胞株强,经DHT诱导后,LRP表达进一步增强。结论雄激素可诱导耐比卡鲁胺前列腺癌细胞肺耐药蛋白(LRP)表达增强。Objective To investigate the regulating effect of androgen on the expression of lung resistance protein （LRP） in bicalutamide-resistant human prostate cancer ceils, and provide a theoretical basis for the further study of prostate cancer pathologically and clinically. Methods The PC-3 and LNCaP cell lines were used to estab- lish bicalutamide-resistant human prostate cancer cell line, which were then induced with dihydrotestosterone （DHT）. The expression of PRP was detected by immunocytochemistry and Western blot. Results The expression of LRP in bicalutamide-resistant human prostate cancer cells was higher than that in human prostate cancer cells. After DHT in- duction, the LRP expression was further enhanced. Conclusion With androgen induction, LRP expression is en- hanced in bicalutamide-resistant human prostate cancer cells.

关 键 词：雄激素 比卡鲁胺 前列腺癌细胞 肺耐药蛋白 耐药性 

分 类 号：R737.25[医药卫生—肿瘤]